Abstract
Patients undergoing allogeneic cord blood transplantation (CBT) benefit from a low risk of graft-versus-host disease (GVHD), but there are still concerns that they be able to recover an effective immune capacity early after transplantation. We investigated the ability to develop in vitro T-lymphocyte-mediated immune response toward human cytomegalovirus and Candida albicans antigens, early and late after transplantation, in children given cord blood transplants from either an HLA-identical sibling or an unrelated donor. Proliferative capacity and frequency of antigen-specific T cells were evaluated; antigen-specific CD4+ T-cell clones were also generated and characterized for T-cell receptor repertoire diversity, cytokine phenotype, and their origin (either from donor or patient). We found that the majority of recipients can develop a specific response to viral or fungal antigens already early after transplantation. Antigen-specific T-cell clones of both donor and recipient origin contributed to the reconstitution of immune response. Antigen-specific T lymphocytes of recipient origin were detected in patients receiving a transplant from a relative, after a chemotherapy-based conditioning regimen, and who did not have GVHD. Our results document, at a clonal level, that after CBT recovery of either polyclonal or pauciclonal T-cell response toward widespread pathogens is prompt, with some patients benefiting from a contribution of recipient-derived cells.
| Original language | English |
|---|---|
| Pages (from-to) | 4322-4329 |
| Number of pages | 8 |
| Journal | Blood |
| Volume | 103 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Jun 1 2004 |
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ASJC Scopus subject areas
- Hematology
Cite this
T lymphocytes of recipient origin may contribute to the recovery of specific immune response toward viruses and fungi in children undergoing cord blood transplantation. / Montagna, Daniela; Locatelli, Franco; Moretta, Antonia; Lisini, Daniela; Previderè, Carlo; Grignani, Pierangela; DeStefano, Piero; Giorgiani, Giovanna; Montini, Enrica; Pagani, Sara; Comoli, Patrizia; Maccario, Rita.
In: Blood, Vol. 103, No. 11, 01.06.2004, p. 4322-4329.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - T lymphocytes of recipient origin may contribute to the recovery of specific immune response toward viruses and fungi in children undergoing cord blood transplantation
AU - Montagna, Daniela
AU - Locatelli, Franco
AU - Moretta, Antonia
AU - Lisini, Daniela
AU - Previderè, Carlo
AU - Grignani, Pierangela
AU - DeStefano, Piero
AU - Giorgiani, Giovanna
AU - Montini, Enrica
AU - Pagani, Sara
AU - Comoli, Patrizia
AU - Maccario, Rita
PY - 2004/6/1
Y1 - 2004/6/1
N2 - Patients undergoing allogeneic cord blood transplantation (CBT) benefit from a low risk of graft-versus-host disease (GVHD), but there are still concerns that they be able to recover an effective immune capacity early after transplantation. We investigated the ability to develop in vitro T-lymphocyte-mediated immune response toward human cytomegalovirus and Candida albicans antigens, early and late after transplantation, in children given cord blood transplants from either an HLA-identical sibling or an unrelated donor. Proliferative capacity and frequency of antigen-specific T cells were evaluated; antigen-specific CD4+ T-cell clones were also generated and characterized for T-cell receptor repertoire diversity, cytokine phenotype, and their origin (either from donor or patient). We found that the majority of recipients can develop a specific response to viral or fungal antigens already early after transplantation. Antigen-specific T-cell clones of both donor and recipient origin contributed to the reconstitution of immune response. Antigen-specific T lymphocytes of recipient origin were detected in patients receiving a transplant from a relative, after a chemotherapy-based conditioning regimen, and who did not have GVHD. Our results document, at a clonal level, that after CBT recovery of either polyclonal or pauciclonal T-cell response toward widespread pathogens is prompt, with some patients benefiting from a contribution of recipient-derived cells.
AB - Patients undergoing allogeneic cord blood transplantation (CBT) benefit from a low risk of graft-versus-host disease (GVHD), but there are still concerns that they be able to recover an effective immune capacity early after transplantation. We investigated the ability to develop in vitro T-lymphocyte-mediated immune response toward human cytomegalovirus and Candida albicans antigens, early and late after transplantation, in children given cord blood transplants from either an HLA-identical sibling or an unrelated donor. Proliferative capacity and frequency of antigen-specific T cells were evaluated; antigen-specific CD4+ T-cell clones were also generated and characterized for T-cell receptor repertoire diversity, cytokine phenotype, and their origin (either from donor or patient). We found that the majority of recipients can develop a specific response to viral or fungal antigens already early after transplantation. Antigen-specific T-cell clones of both donor and recipient origin contributed to the reconstitution of immune response. Antigen-specific T lymphocytes of recipient origin were detected in patients receiving a transplant from a relative, after a chemotherapy-based conditioning regimen, and who did not have GVHD. Our results document, at a clonal level, that after CBT recovery of either polyclonal or pauciclonal T-cell response toward widespread pathogens is prompt, with some patients benefiting from a contribution of recipient-derived cells.
UR - http://www.scopus.com/inward/record.url?scp=2542464929&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2542464929&partnerID=8YFLogxK
U2 - 10.1182/blood-2003-11-4041
DO - 10.1182/blood-2003-11-4041
M3 - Article
C2 - 14764522
AN - SCOPUS:2542464929
VL - 103
SP - 4322
EP - 4329
JO - Blood
JF - Blood
SN - 0006-4971
IS - 11
ER -