T(14;18) translocation in chronic hepatitis C virus infection

Anna Linda Zignego, Francesca Giannelli, Maria Eugenia Marrocchi, Antonio Mazzocca, Clodoveo Ferri, Carlo Giannini, Monica Monti, Patrizio Caini, Giorgio La Villa, Giacomo Laffi, Paolo Gentilini

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Abstract

Pathogenic mechanisms of B-cell lymphoproliferative disorders in chronic hepatitis C virus (HCV) infection are unclear. We studied t(14;18) translocation by polymerase chain reaction in peripheral blood mononuclear cells from 50 patients with HCV-related liver disease (group A), 7 with mixed cryoglobulinemia syndrome (group B), 55 with HCV-negative liver disease (group C), and 30 with HCV-negative chronic rheumatic disorders or chronic infection by nonhepatotropic agents (group D). T(14;18) was significantly more frequent in group A (13/50 patients = 26%) and group B (5/7 = 71.4%) patients than in group C (1/55 = 3.6%) and group D (1/30 = 3.3%) ones. Immunoblot analysis showed bcl-2 over-expression in all t(14;18)positive samples. In group A, 10/13 (77%) patients with t(14;18) and 13/37 (35%) without t(14;18) had serum cryoglobulins in the absence of mixed cryoglobulinemia symptoms (P <.05). These data indicate that t(14;18) and bcl-2 over-expression in lymphoid cells are frequent in chronic HCV infection and suggest that this event may contribute to the pathogenesis of HCV-related lymphoproliferative disorders.

Original languageEnglish
Pages (from-to)474-479
Number of pages6
JournalHepatology
Volume31
Issue number2
Publication statusPublished - 2000

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Chronic Hepatitis C
Virus Diseases
Hepacivirus
Cryoglobulinemia
Lymphoproliferative Disorders
Liver Diseases
Cryoglobulins
Blood Cells
B-Lymphocytes
Lymphocytes
Polymerase Chain Reaction
Infection
Serum

ASJC Scopus subject areas

  • Hepatology

Cite this

Zignego, A. L., Giannelli, F., Marrocchi, M. E., Mazzocca, A., Ferri, C., Giannini, C., ... Gentilini, P. (2000). T(14;18) translocation in chronic hepatitis C virus infection. Hepatology, 31(2), 474-479.

T(14;18) translocation in chronic hepatitis C virus infection. / Zignego, Anna Linda; Giannelli, Francesca; Marrocchi, Maria Eugenia; Mazzocca, Antonio; Ferri, Clodoveo; Giannini, Carlo; Monti, Monica; Caini, Patrizio; La Villa, Giorgio; Laffi, Giacomo; Gentilini, Paolo.

In: Hepatology, Vol. 31, No. 2, 2000, p. 474-479.

Research output: Contribution to journalArticle

Zignego, AL, Giannelli, F, Marrocchi, ME, Mazzocca, A, Ferri, C, Giannini, C, Monti, M, Caini, P, La Villa, G, Laffi, G & Gentilini, P 2000, 'T(14;18) translocation in chronic hepatitis C virus infection', Hepatology, vol. 31, no. 2, pp. 474-479.
Zignego AL, Giannelli F, Marrocchi ME, Mazzocca A, Ferri C, Giannini C et al. T(14;18) translocation in chronic hepatitis C virus infection. Hepatology. 2000;31(2):474-479.
Zignego, Anna Linda ; Giannelli, Francesca ; Marrocchi, Maria Eugenia ; Mazzocca, Antonio ; Ferri, Clodoveo ; Giannini, Carlo ; Monti, Monica ; Caini, Patrizio ; La Villa, Giorgio ; Laffi, Giacomo ; Gentilini, Paolo. / T(14;18) translocation in chronic hepatitis C virus infection. In: Hepatology. 2000 ; Vol. 31, No. 2. pp. 474-479.
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