T2w-MRI signal normalization affects radiomics features reproducibility.

Elisa Scalco, Antonella Belfatto, Alfonso Mastropietro, Tiziana Rancati, Barbara Avuzzi, Antonella Messina, Riccardo Valdagni, Giovanna Rizzo

Research output: Contribution to journalArticlepeer-review


PURPOSE: Despite its increasing application, radiomics has not yet demonstrated a solid reliability, due to the difficulty in replicating analyses. The extraction of radiomic features from clinical MRI (T1w/T2w) presents even more challenges because of the absence of well-defined units (e.g. HU). Some preprocessing steps are required before the estimation of radiomic features and one of this is the intensity normalization, that can be performed using different methods. The aim of this work was to evaluate the effect of three different normalization techniques, applied on T2w-MRI images of the pelvic region, on radiomic features reproducibility. METHODS: T2w-MRI acquired before (MRI1) and 12 months after radiotherapy (MRI2) from 14 patients treated for prostate cancer were considered. Four different conditions were analyzed: (a) the original MRI (NoNorm); (b) MRI normalized by the mean image value (NormMean); (c) MRI normalized by the mean value of the urine in the bladder (NormROI); (d) MRI normalized by the histogram-matching method (NormHM). Ninety-one radiomic features were extracted from three organs of interest (prostate, internal obturator muscles and bulb) at both time-points and on each image discretized using a fixed bin-width approach and the difference between the two time-points was calculated (Δfeature). To estimate the effect of normalization methods on the reproducibility of radiomic features, ICC was calculated in three analyses: (a) considering the features extracted on MRI2 in the four conditions together and considering the influence of each method separately, with respect to NoNorm; (b) considering the features extracted on MRI2 in the four conditions with respect to the inter-observer variability in region of interest (ROI) contouring, considering also the effect of the discretization approach; (c) considering Δfeature to evaluate if some indices can recover some consistency when differences are calculated. RESULTS: Nearly 60ICC textless 0.5) on MRI2 and the method that most affected features reliability was NormROI (average ICC of 0.45). The other two methods were similar, except for first-order features, where NormHM outperformed NormMean (average ICC = 0.33 and 0.76 for NormMean and NormHM, respectively). In the inter-observer setting, the number of reproducible features varied in the three structures, being higher in the prostate than in the penile bulb and in the obturators. The analysis on Δfeature highlighted that more than 60% of the features were not consistent with respect to the normalization method and confirmed the high reproducibility of the features between NormMean and NormHM, whereas NormROI was the less reproducible method. CONCLUSIONS: The normalization process impacts the reproducibility of radiomic features, both in terms of changes in the image information content and in the inter-observer setting. Among the considered methods, NormMean and NormHM seem to provide the most reproducible features with respect to the original image and also between themselves, whereas NormROI generates less reproducible features. Only a very small subset of feature remained reproducible and independent in any tested condition, regardless the ROI and the adopted algorithm: skewness or kurtosis, correlation and one among Imc2, Idmn and Idn from GLCM group.
Original languageEnglish
JournalMedical Physics
Issue number4
Publication statusPublished - Apr 1 2020

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