Talampanel reduces the level of motoneuronal calcium in transgenic mutant SOD1 mice only if applied presymptomatically

Melinda Paizs, Massimo Tortarolo, Caterina Bendotti, József I. Engelhardt, László Siklós

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We tested the efficacy of treatment with talampanel in a mutant SOD1 mouse model of ALS by measuring intracellular calcium levels and loss of spinal motor neurons. We intended to mimic the clinical study; hence, treatment was started when the clinical symptoms were already present. The data were compared with the results of similar treatment started at a presymptomatic stage. Transgenic and wild-type mice were treated either with talampanel or with vehicle, starting in presymptomatic or symptomatic stages. The density of motor neurons was determined by the physical disector, and their intracellular calcium level was assayed electron microscopically. Results showed that motor neurons in the SOD1 mice exhibited an elevated calcium level, which could be reduced, but not restored, with talampanel only when the treatment was started presymptomatically. Treatment in either presymptomatic or symptomatic stages failed to rescue the motor neurons. We conclude that talampanel reduces motoneuronal calcium in a mouse model of ALS, but its efficacy declines as the disease progresses, suggesting that medication initiation in the earlier stages of the disease might be more effective.

Original languageEnglish
Pages (from-to)340-344
Number of pages5
JournalAmyotrophic Lateral Sclerosis
Volume12
Issue number5
DOIs
Publication statusPublished - Sep 2011

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GYKI 53405
Motor Neurons
Calcium
Transgenic Mice
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Keywords

  • AMPA receptor
  • Intracellular calcium
  • Motor neuron
  • MSOD1 model
  • Talampanel

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology

Cite this

Talampanel reduces the level of motoneuronal calcium in transgenic mutant SOD1 mice only if applied presymptomatically. / Paizs, Melinda; Tortarolo, Massimo; Bendotti, Caterina; Engelhardt, József I.; Siklós, László.

In: Amyotrophic Lateral Sclerosis, Vol. 12, No. 5, 09.2011, p. 340-344.

Research output: Contribution to journalArticle

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