Tamoxifen induced membrane alterations in human breast cancer cells

G. Sica, C. Natoli, P. Marchetti, S. Piperno, S. Iacobelli

Research output: Contribution to journalArticle

Abstract

It was previously shown that proliferation of CG-5 cells, an estrogen supersensitive variant of the MCF-7 cell line, is inhibited by tamoxifen starting three days after addition to culture medium. In this paper we report here on the effect of tamoxifen on CG-5 cell membrane morphology using scanning electron microscope. The addition of the antiestrogen to CG-5 cells causes marked alterations of cell surface, consisting of reduction in the length and the number of microvilli. Moreover, cells appear flattened and of reduced thickness. These modifications are more pronounced in cells plated at low density, but they are observed also in nearly confluent monolayers. In this case alterations are more evident in the center of cell islands, while at the periphery there is a certain mobility as reflected by the persistence of few microvilli and philopodia. These early effects of tamoxifen on cell membrane raise the possibility that parallel modifications in membrane function may occur which are responsible for alterations impairing transport mechanisms.

Original languageEnglish
Pages (from-to)425-428
Number of pages4
JournalJournal of Steroid Biochemistry
Volume20
Issue number1
DOIs
Publication statusPublished - 1984

Fingerprint

Tamoxifen
Cells
Cell membranes
Breast Neoplasms
Membranes
Estrogen Receptor Modulators
Microvilli
Culture Media
Monolayers
Estrogens
Electron microscopes
Cell Membrane
Scanning
MCF-7 Cells
Electrons
Cell Line

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Tamoxifen induced membrane alterations in human breast cancer cells. / Sica, G.; Natoli, C.; Marchetti, P.; Piperno, S.; Iacobelli, S.

In: Journal of Steroid Biochemistry, Vol. 20, No. 1, 1984, p. 425-428.

Research output: Contribution to journalArticle

Sica, G, Natoli, C, Marchetti, P, Piperno, S & Iacobelli, S 1984, 'Tamoxifen induced membrane alterations in human breast cancer cells', Journal of Steroid Biochemistry, vol. 20, no. 1, pp. 425-428. https://doi.org/10.1016/0022-4731(84)90246-2
Sica, G. ; Natoli, C. ; Marchetti, P. ; Piperno, S. ; Iacobelli, S. / Tamoxifen induced membrane alterations in human breast cancer cells. In: Journal of Steroid Biochemistry. 1984 ; Vol. 20, No. 1. pp. 425-428.
@article{87bcd525b09f4e4aa74728395cb12092,
title = "Tamoxifen induced membrane alterations in human breast cancer cells",
abstract = "It was previously shown that proliferation of CG-5 cells, an estrogen supersensitive variant of the MCF-7 cell line, is inhibited by tamoxifen starting three days after addition to culture medium. In this paper we report here on the effect of tamoxifen on CG-5 cell membrane morphology using scanning electron microscope. The addition of the antiestrogen to CG-5 cells causes marked alterations of cell surface, consisting of reduction in the length and the number of microvilli. Moreover, cells appear flattened and of reduced thickness. These modifications are more pronounced in cells plated at low density, but they are observed also in nearly confluent monolayers. In this case alterations are more evident in the center of cell islands, while at the periphery there is a certain mobility as reflected by the persistence of few microvilli and philopodia. These early effects of tamoxifen on cell membrane raise the possibility that parallel modifications in membrane function may occur which are responsible for alterations impairing transport mechanisms.",
author = "G. Sica and C. Natoli and P. Marchetti and S. Piperno and S. Iacobelli",
year = "1984",
doi = "10.1016/0022-4731(84)90246-2",
language = "English",
volume = "20",
pages = "425--428",
journal = "Journal of Steroid Biochemistry",
issn = "0022-4731",
publisher = "Pergamon Press Ltd.",
number = "1",

}

TY - JOUR

T1 - Tamoxifen induced membrane alterations in human breast cancer cells

AU - Sica, G.

AU - Natoli, C.

AU - Marchetti, P.

AU - Piperno, S.

AU - Iacobelli, S.

PY - 1984

Y1 - 1984

N2 - It was previously shown that proliferation of CG-5 cells, an estrogen supersensitive variant of the MCF-7 cell line, is inhibited by tamoxifen starting three days after addition to culture medium. In this paper we report here on the effect of tamoxifen on CG-5 cell membrane morphology using scanning electron microscope. The addition of the antiestrogen to CG-5 cells causes marked alterations of cell surface, consisting of reduction in the length and the number of microvilli. Moreover, cells appear flattened and of reduced thickness. These modifications are more pronounced in cells plated at low density, but they are observed also in nearly confluent monolayers. In this case alterations are more evident in the center of cell islands, while at the periphery there is a certain mobility as reflected by the persistence of few microvilli and philopodia. These early effects of tamoxifen on cell membrane raise the possibility that parallel modifications in membrane function may occur which are responsible for alterations impairing transport mechanisms.

AB - It was previously shown that proliferation of CG-5 cells, an estrogen supersensitive variant of the MCF-7 cell line, is inhibited by tamoxifen starting three days after addition to culture medium. In this paper we report here on the effect of tamoxifen on CG-5 cell membrane morphology using scanning electron microscope. The addition of the antiestrogen to CG-5 cells causes marked alterations of cell surface, consisting of reduction in the length and the number of microvilli. Moreover, cells appear flattened and of reduced thickness. These modifications are more pronounced in cells plated at low density, but they are observed also in nearly confluent monolayers. In this case alterations are more evident in the center of cell islands, while at the periphery there is a certain mobility as reflected by the persistence of few microvilli and philopodia. These early effects of tamoxifen on cell membrane raise the possibility that parallel modifications in membrane function may occur which are responsible for alterations impairing transport mechanisms.

UR - http://www.scopus.com/inward/record.url?scp=0021331846&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021331846&partnerID=8YFLogxK

U2 - 10.1016/0022-4731(84)90246-2

DO - 10.1016/0022-4731(84)90246-2

M3 - Article

C2 - 6708527

AN - SCOPUS:0021331846

VL - 20

SP - 425

EP - 428

JO - Journal of Steroid Biochemistry

JF - Journal of Steroid Biochemistry

SN - 0022-4731

IS - 1

ER -