Abstract
p73 and tau both play roles in neurodevelopment and neurodegeneration. In this pilot study we show by Western blotting that TAp73α induces phosphorylation of human 2N4R tau at threonine-205 and at the PHF-1 epitope (serine366/serine404) in HEK293a cells. Neither the dominant negative isoform, ΔNp73, nor a transcriptionally inactive mutant TAp73αR292H altered tau phosphorylation indicating that tau phosphorylation is dependent on the transcriptional activity of TAp73α. Consistent with this, confocal microscopy revealed that tau and TAp73α were spatially separated within the cell; tau being located in the cytoskeletal compartment whilst TAp73α was found in the nucleus. These findings have ramifications for microtubule dynamics associated with axonal growth during development and for neuronal death associated with Alzheimer's disease and other tauopathies.
| Original language | English |
|---|---|
| Pages (from-to) | 30-34 |
| Number of pages | 5 |
| Journal | Neuroscience Letters |
| Volume | 401 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Jun 19 2006 |
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Keywords
- Alzheimer's disease
- Microtubules
- Neurodevelopment
- p53
- p73
- Tau
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
TAp73α induces tau phosphorylation in HEK293a cells via a transcription-dependent mechanism. / Hooper, Claudie; Killick, Richard; Tavassoli, Mahvash; Melino, Gerry; Lovestone, Simon.
In: Neuroscience Letters, Vol. 401, No. 1-2, 19.06.2006, p. 30-34.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - TAp73α induces tau phosphorylation in HEK293a cells via a transcription-dependent mechanism
AU - Hooper, Claudie
AU - Killick, Richard
AU - Tavassoli, Mahvash
AU - Melino, Gerry
AU - Lovestone, Simon
PY - 2006/6/19
Y1 - 2006/6/19
N2 - p73 and tau both play roles in neurodevelopment and neurodegeneration. In this pilot study we show by Western blotting that TAp73α induces phosphorylation of human 2N4R tau at threonine-205 and at the PHF-1 epitope (serine366/serine404) in HEK293a cells. Neither the dominant negative isoform, ΔNp73, nor a transcriptionally inactive mutant TAp73αR292H altered tau phosphorylation indicating that tau phosphorylation is dependent on the transcriptional activity of TAp73α. Consistent with this, confocal microscopy revealed that tau and TAp73α were spatially separated within the cell; tau being located in the cytoskeletal compartment whilst TAp73α was found in the nucleus. These findings have ramifications for microtubule dynamics associated with axonal growth during development and for neuronal death associated with Alzheimer's disease and other tauopathies.
AB - p73 and tau both play roles in neurodevelopment and neurodegeneration. In this pilot study we show by Western blotting that TAp73α induces phosphorylation of human 2N4R tau at threonine-205 and at the PHF-1 epitope (serine366/serine404) in HEK293a cells. Neither the dominant negative isoform, ΔNp73, nor a transcriptionally inactive mutant TAp73αR292H altered tau phosphorylation indicating that tau phosphorylation is dependent on the transcriptional activity of TAp73α. Consistent with this, confocal microscopy revealed that tau and TAp73α were spatially separated within the cell; tau being located in the cytoskeletal compartment whilst TAp73α was found in the nucleus. These findings have ramifications for microtubule dynamics associated with axonal growth during development and for neuronal death associated with Alzheimer's disease and other tauopathies.
KW - Alzheimer's disease
KW - Microtubules
KW - Neurodevelopment
KW - p53
KW - p73
KW - Tau
UR - http://www.scopus.com/inward/record.url?scp=33646683586&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646683586&partnerID=8YFLogxK
U2 - 10.1016/j.neulet.2006.02.082
DO - 10.1016/j.neulet.2006.02.082
M3 - Article
C2 - 16580132
AN - SCOPUS:33646683586
VL - 401
SP - 30
EP - 34
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1-2
ER -