Abstract
Studies on oocyte transcriptome are important to understand the biological pathways involved in oogenesis, totipotence and early embryonic development. Moreover, genes regulating physiological pathways in gametes could represent potential candidates for reproductive disorders. In addition to oocyte specific transcription factors, also the members of the p53 family could be etiologically involved due to their biological functions. In fact, their role in the control of cell cycle, apoptosis and germ-line genome stability is well known. Female reproductive aging is one of the causes of fertility reduction and it is often associated with egg aneuploidy increase. In order to verify the potential involvement of p73 in reproductive aging, we determined its expression in single mature MII oocytes from two groups of women, younger than 35 or older than 38 y, respectively. We found that TAp73 isoforms are downregulated in oocytes from women older than 38 y. We confirmed these data in pools of mouse oocytes. TAp73 downregulation in oocytes from women of advanced reproductive age could explain both the reduction of fertility and the increase of newborns with chromosomal abnormalities.
| Original language | English |
|---|---|
| Pages (from-to) | 3253-3256 |
| Number of pages | 4 |
| Journal | Cell Cycle |
| Volume | 10 |
| Issue number | 19 |
| DOIs | |
| Publication status | Published - Oct 1 2011 |
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Keywords
- Aneuploidy
- Human oocyte
- Maternal mRNAs
- p73
- Reproductive aging
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
- Developmental Biology
Cite this
TAp73 is downregulated in oocytes from women of advanced reproductive age. / Guglielmino, Maria Rosa; Santonocito, Manuela; Vento, Marilena; Ragusa, Marco; Barbagallo, Davide; Borzì, Placido; Casciano, Ida; Banelli, Barbara; Barbieri, Ottavia; Astigiano, Simonetta; Scollo, Paolo; Romani, Massimo; Purrello, Michele; Di Pietro, Cinzia.
In: Cell Cycle, Vol. 10, No. 19, 01.10.2011, p. 3253-3256.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - TAp73 is downregulated in oocytes from women of advanced reproductive age
AU - Guglielmino, Maria Rosa
AU - Santonocito, Manuela
AU - Vento, Marilena
AU - Ragusa, Marco
AU - Barbagallo, Davide
AU - Borzì, Placido
AU - Casciano, Ida
AU - Banelli, Barbara
AU - Barbieri, Ottavia
AU - Astigiano, Simonetta
AU - Scollo, Paolo
AU - Romani, Massimo
AU - Purrello, Michele
AU - Di Pietro, Cinzia
PY - 2011/10/1
Y1 - 2011/10/1
N2 - Studies on oocyte transcriptome are important to understand the biological pathways involved in oogenesis, totipotence and early embryonic development. Moreover, genes regulating physiological pathways in gametes could represent potential candidates for reproductive disorders. In addition to oocyte specific transcription factors, also the members of the p53 family could be etiologically involved due to their biological functions. In fact, their role in the control of cell cycle, apoptosis and germ-line genome stability is well known. Female reproductive aging is one of the causes of fertility reduction and it is often associated with egg aneuploidy increase. In order to verify the potential involvement of p73 in reproductive aging, we determined its expression in single mature MII oocytes from two groups of women, younger than 35 or older than 38 y, respectively. We found that TAp73 isoforms are downregulated in oocytes from women older than 38 y. We confirmed these data in pools of mouse oocytes. TAp73 downregulation in oocytes from women of advanced reproductive age could explain both the reduction of fertility and the increase of newborns with chromosomal abnormalities.
AB - Studies on oocyte transcriptome are important to understand the biological pathways involved in oogenesis, totipotence and early embryonic development. Moreover, genes regulating physiological pathways in gametes could represent potential candidates for reproductive disorders. In addition to oocyte specific transcription factors, also the members of the p53 family could be etiologically involved due to their biological functions. In fact, their role in the control of cell cycle, apoptosis and germ-line genome stability is well known. Female reproductive aging is one of the causes of fertility reduction and it is often associated with egg aneuploidy increase. In order to verify the potential involvement of p73 in reproductive aging, we determined its expression in single mature MII oocytes from two groups of women, younger than 35 or older than 38 y, respectively. We found that TAp73 isoforms are downregulated in oocytes from women older than 38 y. We confirmed these data in pools of mouse oocytes. TAp73 downregulation in oocytes from women of advanced reproductive age could explain both the reduction of fertility and the increase of newborns with chromosomal abnormalities.
KW - Aneuploidy
KW - Human oocyte
KW - Maternal mRNAs
KW - p73
KW - Reproductive aging
UR - http://www.scopus.com/inward/record.url?scp=80053456743&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80053456743&partnerID=8YFLogxK
U2 - 10.4161/cc.10.19.17585
DO - 10.4161/cc.10.19.17585
M3 - Article
C2 - 21946516
AN - SCOPUS:80053456743
VL - 10
SP - 3253
EP - 3256
JO - Cell Cycle
JF - Cell Cycle
SN - 1538-4101
IS - 19
ER -