TY - JOUR
T1 - TAp73 is required for macrophage-mediated innate immunity and the resolution of inflammatory responses
AU - Tomasini, R.
AU - Secq, V.
AU - Pouyet, L.
AU - Thakur, A. K.
AU - Wilhelm, M.
AU - Nigri, J.
AU - Vasseur, S.
AU - Berthezene, P.
AU - Calvo, E.
AU - Melino, G.
AU - Mak, T. W.
AU - Iovanna, J. L.
PY - 2013/2
Y1 - 2013/2
N2 - The multiple isoforms of p73, a member of the p53 family, share the ability to modulate p53 activities but also have unique properties, leading to a complex and poorly understood functional network. In vivo, p73 isoforms have been implicated in tumor suppression (TAp73-/- mice), DNA damage (ΔNp73-/- mice) and development (p73-/- mice). In this study, we investigated whether TAp73 contributes to innate immunity and septic shock. In response to a lethal lipopolysaccharide (LPS) challenge, TAp73-/- mice showed higher blood levels of proinflammatory cytokines and greater mortality than their wild-type littermates. In vitro, TAp73 -/- macrophages exhibited elevated production of tumor necrosis factor alpha, interleukin-6 and macrophage inflammatory protein-2 as well as prolonged survival, decreased phagocytosis and increased major histocompatibility complex class II expression. Mice depleted of endogenous macrophages and reconstituted with TAp73-/- macrophages showed increased sensitivity to LPS challenge. These results suggest that macrophage polarization is altered in the absence of TAp73 such that maintenance of the M1 effector phenotype is prolonged at the expense of the M2 phenotype, thus impairing resolution of the inflammatory response. Our data indicate that TAp73 has a role in macrophage polarization and innate immunity, enhancing the action field of this important regulatory molecule.
AB - The multiple isoforms of p73, a member of the p53 family, share the ability to modulate p53 activities but also have unique properties, leading to a complex and poorly understood functional network. In vivo, p73 isoforms have been implicated in tumor suppression (TAp73-/- mice), DNA damage (ΔNp73-/- mice) and development (p73-/- mice). In this study, we investigated whether TAp73 contributes to innate immunity and septic shock. In response to a lethal lipopolysaccharide (LPS) challenge, TAp73-/- mice showed higher blood levels of proinflammatory cytokines and greater mortality than their wild-type littermates. In vitro, TAp73 -/- macrophages exhibited elevated production of tumor necrosis factor alpha, interleukin-6 and macrophage inflammatory protein-2 as well as prolonged survival, decreased phagocytosis and increased major histocompatibility complex class II expression. Mice depleted of endogenous macrophages and reconstituted with TAp73-/- macrophages showed increased sensitivity to LPS challenge. These results suggest that macrophage polarization is altered in the absence of TAp73 such that maintenance of the M1 effector phenotype is prolonged at the expense of the M2 phenotype, thus impairing resolution of the inflammatory response. Our data indicate that TAp73 has a role in macrophage polarization and innate immunity, enhancing the action field of this important regulatory molecule.
KW - apoptosis
KW - inflammation
KW - M1/M2 polarization
KW - macrophage
KW - p73
UR - http://www.scopus.com/inward/record.url?scp=84872295543&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84872295543&partnerID=8YFLogxK
U2 - 10.1038/cdd.2012.123
DO - 10.1038/cdd.2012.123
M3 - Article
C2 - 22976836
AN - SCOPUS:84872295543
VL - 20
SP - 293
EP - 301
JO - Cell Death and Differentiation
JF - Cell Death and Differentiation
SN - 1350-9047
IS - 2
ER -