TAp73 is required for macrophage-mediated innate immunity and the resolution of inflammatory responses

R. Tomasini, V. Secq, L. Pouyet, A. K. Thakur, M. Wilhelm, J. Nigri, S. Vasseur, P. Berthezene, E. Calvo, G. Melino, T. W. Mak, J. L. Iovanna

Research output: Contribution to journalArticle

Abstract

The multiple isoforms of p73, a member of the p53 family, share the ability to modulate p53 activities but also have unique properties, leading to a complex and poorly understood functional network. In vivo, p73 isoforms have been implicated in tumor suppression (TAp73-/- mice), DNA damage (ΔNp73-/- mice) and development (p73-/- mice). In this study, we investigated whether TAp73 contributes to innate immunity and septic shock. In response to a lethal lipopolysaccharide (LPS) challenge, TAp73-/- mice showed higher blood levels of proinflammatory cytokines and greater mortality than their wild-type littermates. In vitro, TAp73 -/- macrophages exhibited elevated production of tumor necrosis factor alpha, interleukin-6 and macrophage inflammatory protein-2 as well as prolonged survival, decreased phagocytosis and increased major histocompatibility complex class II expression. Mice depleted of endogenous macrophages and reconstituted with TAp73-/- macrophages showed increased sensitivity to LPS challenge. These results suggest that macrophage polarization is altered in the absence of TAp73 such that maintenance of the M1 effector phenotype is prolonged at the expense of the M2 phenotype, thus impairing resolution of the inflammatory response. Our data indicate that TAp73 has a role in macrophage polarization and innate immunity, enhancing the action field of this important regulatory molecule.

Original languageEnglish
Pages (from-to)293-301
Number of pages9
JournalCell Death and Differentiation
Volume20
Issue number2
DOIs
Publication statusPublished - Feb 2013

Keywords

  • apoptosis
  • inflammation
  • M1/M2 polarization
  • macrophage
  • p73

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Fingerprint Dive into the research topics of 'TAp73 is required for macrophage-mediated innate immunity and the resolution of inflammatory responses'. Together they form a unique fingerprint.

  • Cite this

    Tomasini, R., Secq, V., Pouyet, L., Thakur, A. K., Wilhelm, M., Nigri, J., Vasseur, S., Berthezene, P., Calvo, E., Melino, G., Mak, T. W., & Iovanna, J. L. (2013). TAp73 is required for macrophage-mediated innate immunity and the resolution of inflammatory responses. Cell Death and Differentiation, 20(2), 293-301. https://doi.org/10.1038/cdd.2012.123