Target epitopes of myelin basic protein specific T cell lines in multiple sclerosis

G. Ristori, M. Salvetti, C. Buttinelli, M. Falcone, S. Trabace, C. Fieschi

Research output: Contribution to journalArticlepeer-review

Abstract

A highly efficient new method for the generation of antigen specific T cell lines (TCL) is now available. By this method we established 134 myelin basic protein (MBP) TCL from the peripheral blood of 9 patients with definite multiple sclerosis (n=69) and 8 healthy donors (n=65). The yield of MBP reactive TCL in the two groups was comparable. So far 22 MBP specific TCL from 7 patients and 24 from 7 healthy individuals have been tested for their proliferative response to a panel of four synthetic peptides representing MBP residues 7-26, 80-99, 139-153 and 148-162. Although the peptide sequences did not encompass the whole MBP, the pattern of reactivity to these peptides in patients and controls seems to be similar. Further, when multiple TCL from the same donor were analysed, no dominant recognition emerged.

Original languageEnglish
Pages (from-to)139-143
Number of pages5
JournalItalian Journal of Neurological Sciences
Volume14
Issue number2
DOIs
Publication statusPublished - Mar 1993

Keywords

  • Multiple sclerosis
  • myelin basic protein
  • T lymphocytes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology

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