Abstract
Formulations of antisense oligonucleotides (asODNs) against c-myb or c-myc protooncogenes have been prepared by a new technique that sequesters cationic lipid in the interior of a lipid particle. This technique results in high loading efficiency for the asODNs, small particle size and good stability. When targeted against melanoma cells or neuroblastoma cells via anti-GD2 coupled at the particle surface, increased cell binding to the cells could be demonstrated. Targeted formulations showed greater inhibition of cell proliferation compared to non-targeted formulations or free drug. Inhibition of cell proliferation was demonstrated to be due to down-regulation of c-myb or c-myc protein expression. The formulations have long-circulation times in vivo, and evaluation for in vivo antitumor activity is currently underway.
Original language | English |
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Pages (from-to) | 69-75 |
Number of pages | 7 |
Journal | Journal of Controlled Release |
Volume | 74 |
Issue number | 1-3 |
DOIs | |
Publication status | Published - Jul 6 2001 |
Keywords
- Anti-GD
- Antisense oligonucleotides
- Liposomes
- Melanoma
- Targeted drug delivery
ASJC Scopus subject areas
- Pharmaceutical Science