Targeted dual-color silica nanoparticles provide univocal identification of micrometastases in preclinical models of colorectal cancer

Marco Soster, Riccardo Juris, Sara Bonacchi, Damiano Genovese, Marco Montalti, Enrico Rampazzo, Nelsi Zaccheroni, Paolo Garagnani, Federico Bussolino, Luca Prodi, Serena Marchiò

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background and methods: Despite the recent introduction of targeted bio-drugs, the scarcity of successful therapeutic options for advanced colorectal cancer remains a limiting factor in patient management. The efficacy of curative surgical interventions can only be extended through earlier detection of metastatic foci, which is dependent on both the sensitivity and specificity of the diagnostic tools. Results: We propose a high-performance imaging platform based on silica-poly(ethylene glycol) nanoparticles doped with rhodamine B and cyanine 5. Simultaneous detection of these dyes is the basis for background subtraction and signal amplification, thus providing high-sensitivity imaging. The functionalization of poly(ethylene glycol) tails on the external face of the nanoparticles with metastasis-specific peptides guarantees their homing to and accumulation at target tissues, resulting in specific visualization, even of submillimetric metastases. Conclusions: The results reported here demonstrate that our rationally designed modular nanosystems have the ability to produce a breakthrough in the detection of micrometastases for subsequent translation to clinics in the immediate future.

Original languageEnglish
Pages (from-to)4797-4807
Number of pages11
JournalInternational Journal of Nanomedicine
Volume7
DOIs
Publication statusPublished - 2012

Fingerprint

Neoplasm Micrometastasis
rhodamine B
Ethylene Glycol
Silicon Dioxide
Nanoparticles
Polyethylene glycols
Colorectal Neoplasms
Identification (control systems)
Color
Silica
Neoplasm Metastasis
Nanosystems
Imaging techniques
Peptides
Amplification
Coloring Agents
Visualization
Dyes
Tissue
Sensitivity and Specificity

Keywords

  • Colorectal cancer
  • Imaging platform
  • Luminescent targeting
  • Micrometastasis
  • Peptide targeting

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Organic Chemistry
  • Drug Discovery

Cite this

Targeted dual-color silica nanoparticles provide univocal identification of micrometastases in preclinical models of colorectal cancer. / Soster, Marco; Juris, Riccardo; Bonacchi, Sara; Genovese, Damiano; Montalti, Marco; Rampazzo, Enrico; Zaccheroni, Nelsi; Garagnani, Paolo; Bussolino, Federico; Prodi, Luca; Marchiò, Serena.

In: International Journal of Nanomedicine, Vol. 7, 2012, p. 4797-4807.

Research output: Contribution to journalArticle

Soster, Marco ; Juris, Riccardo ; Bonacchi, Sara ; Genovese, Damiano ; Montalti, Marco ; Rampazzo, Enrico ; Zaccheroni, Nelsi ; Garagnani, Paolo ; Bussolino, Federico ; Prodi, Luca ; Marchiò, Serena. / Targeted dual-color silica nanoparticles provide univocal identification of micrometastases in preclinical models of colorectal cancer. In: International Journal of Nanomedicine. 2012 ; Vol. 7. pp. 4797-4807.
@article{e5b1ec60c7cc42c6ab72711fba53f694,
title = "Targeted dual-color silica nanoparticles provide univocal identification of micrometastases in preclinical models of colorectal cancer",
abstract = "Background and methods: Despite the recent introduction of targeted bio-drugs, the scarcity of successful therapeutic options for advanced colorectal cancer remains a limiting factor in patient management. The efficacy of curative surgical interventions can only be extended through earlier detection of metastatic foci, which is dependent on both the sensitivity and specificity of the diagnostic tools. Results: We propose a high-performance imaging platform based on silica-poly(ethylene glycol) nanoparticles doped with rhodamine B and cyanine 5. Simultaneous detection of these dyes is the basis for background subtraction and signal amplification, thus providing high-sensitivity imaging. The functionalization of poly(ethylene glycol) tails on the external face of the nanoparticles with metastasis-specific peptides guarantees their homing to and accumulation at target tissues, resulting in specific visualization, even of submillimetric metastases. Conclusions: The results reported here demonstrate that our rationally designed modular nanosystems have the ability to produce a breakthrough in the detection of micrometastases for subsequent translation to clinics in the immediate future.",
keywords = "Colorectal cancer, Imaging platform, Luminescent targeting, Micrometastasis, Peptide targeting",
author = "Marco Soster and Riccardo Juris and Sara Bonacchi and Damiano Genovese and Marco Montalti and Enrico Rampazzo and Nelsi Zaccheroni and Paolo Garagnani and Federico Bussolino and Luca Prodi and Serena Marchi{\`o}",
year = "2012",
doi = "10.2147/IJN.S33825",
language = "English",
volume = "7",
pages = "4797--4807",
journal = "International Journal of Nanomedicine",
issn = "1176-9114",
publisher = "Dove Medical Press Ltd.",

}

TY - JOUR

T1 - Targeted dual-color silica nanoparticles provide univocal identification of micrometastases in preclinical models of colorectal cancer

AU - Soster, Marco

AU - Juris, Riccardo

AU - Bonacchi, Sara

AU - Genovese, Damiano

AU - Montalti, Marco

AU - Rampazzo, Enrico

AU - Zaccheroni, Nelsi

AU - Garagnani, Paolo

AU - Bussolino, Federico

AU - Prodi, Luca

AU - Marchiò, Serena

PY - 2012

Y1 - 2012

N2 - Background and methods: Despite the recent introduction of targeted bio-drugs, the scarcity of successful therapeutic options for advanced colorectal cancer remains a limiting factor in patient management. The efficacy of curative surgical interventions can only be extended through earlier detection of metastatic foci, which is dependent on both the sensitivity and specificity of the diagnostic tools. Results: We propose a high-performance imaging platform based on silica-poly(ethylene glycol) nanoparticles doped with rhodamine B and cyanine 5. Simultaneous detection of these dyes is the basis for background subtraction and signal amplification, thus providing high-sensitivity imaging. The functionalization of poly(ethylene glycol) tails on the external face of the nanoparticles with metastasis-specific peptides guarantees their homing to and accumulation at target tissues, resulting in specific visualization, even of submillimetric metastases. Conclusions: The results reported here demonstrate that our rationally designed modular nanosystems have the ability to produce a breakthrough in the detection of micrometastases for subsequent translation to clinics in the immediate future.

AB - Background and methods: Despite the recent introduction of targeted bio-drugs, the scarcity of successful therapeutic options for advanced colorectal cancer remains a limiting factor in patient management. The efficacy of curative surgical interventions can only be extended through earlier detection of metastatic foci, which is dependent on both the sensitivity and specificity of the diagnostic tools. Results: We propose a high-performance imaging platform based on silica-poly(ethylene glycol) nanoparticles doped with rhodamine B and cyanine 5. Simultaneous detection of these dyes is the basis for background subtraction and signal amplification, thus providing high-sensitivity imaging. The functionalization of poly(ethylene glycol) tails on the external face of the nanoparticles with metastasis-specific peptides guarantees their homing to and accumulation at target tissues, resulting in specific visualization, even of submillimetric metastases. Conclusions: The results reported here demonstrate that our rationally designed modular nanosystems have the ability to produce a breakthrough in the detection of micrometastases for subsequent translation to clinics in the immediate future.

KW - Colorectal cancer

KW - Imaging platform

KW - Luminescent targeting

KW - Micrometastasis

KW - Peptide targeting

UR - http://www.scopus.com/inward/record.url?scp=84870354922&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870354922&partnerID=8YFLogxK

U2 - 10.2147/IJN.S33825

DO - 10.2147/IJN.S33825

M3 - Article

VL - 7

SP - 4797

EP - 4807

JO - International Journal of Nanomedicine

JF - International Journal of Nanomedicine

SN - 1176-9114

ER -