Targeted expression of RALT in mouse skin inhibits epidermal growth factor receptor signalling and generates a Waved-like phenotype

Costanza Ballarò, Sara Ceccarelli, Cecilia Tiveron, Laura Tatangelo, Anna Maria Salvatore, Oreste Segatto, Stefano Alemà

Research output: Contribution to journalArticle

Abstract

Although it has been clearly established that negative feedback loops have a fundamental role in the regulation of epidermal growth factor receptor (EGFR) signalling in flies, their role in the regulation of mammalian EGFR has been inferred only recently from in vitro studies. Here, we report on the forced expression of RALT/MIG-6, a negative feedback regulator of ErbB receptors, in mouse skin. A RALT transgene driven by the K14 promoter generated a dose-dependent phenotype resembling that caused by hypomorphic and antimorphic Egfr alleles - that is, wavy coat, curly whiskers and open eyes at birth. Ex vivo keratinocytes from K14-RALT mice showed reduced biochemical and biological responses when stimulated by ErbB ligands. Conversely, knockdown of RALT by RNA interference enhanced ErbB mitogenic signalling. Thus, RALT behaves as a suppressor of EGFR signalling in mouse skin.

Original languageEnglish
Pages (from-to)755-761
Number of pages7
JournalEMBO Reports
Volume6
Issue number8
DOIs
Publication statusPublished - Aug 2005

Keywords

  • ErbB
  • Feedback inhibition
  • Interference
  • Keratinocyte
  • RALT
  • RNA

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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