Targeting heat shock proteins on cancer cells

Selection, characterization, and cell-penetrating properties of a peptidic GRP78 ligand

Youngsoo Kim, Antonietta M. Lillo, Sebastian C J Steiniger, Ying Liu, Carlo Ballatore, Andrea Anichini, Roberta Mortarini, Gunnar F. Kaufmann, Bin Zhou, Brunhilde Felding-Habermann, Kim D. Janda

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Peptidic ligands can be used for specific cell targeting and the delivery of payloads into the target cell. Here we describe the screening of a pool of cyclic peptide phage display libraries using whole-cell panning against human melanoma cell line Me6652/4. This strategy resulted in the selection of the cyclic 13-mer Pep42, CTVALPGGYVRVC, which showed preferential internalization into melanoma cell line Me6652/4 versus the reference cell line Me6652/56. This translocation is a receptor-mediated process that does not require electrostatic interactions nor does it involve transfer to the lysosomal compartment. The cellular receptor for Pep42 was identified as the surface membrane form of glucose-regulated protein 78 (GRP78), a member of the heat shock protein family and a marker on malignant cancer cells. The cellular uptake and intracellular trafficking of Pep42-Quantum Dot conjugates was monitored by confocal laser microscopy, and colocalization within the endoplasmic reticulum was observed. The uptake of Pep42 could be blocked by a monoclonal antibody against the identified receptor. Furthermore, Pep42 was shown to target specifically GRP78-expressing cancer cells. The in vitro cytotoxicity of a Pep42-Taxol conjugate was evaluated by flow cytometry wherein the conjugate was shown to induce apoptosis and was more effective in promoting programmed cell death in Me6652/4 cells. In summary, the data presented suggest that cyclic peptide Pep42 might be a powerful tool in the construction of drug conjugates designed to selectively kill malignant cancer cells.

Original languageEnglish
Pages (from-to)9434-9444
Number of pages11
JournalBiochemistry
Volume45
Issue number31
DOIs
Publication statusPublished - Aug 8 2006

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Heat-Shock Proteins
Cells
Ligands
Neoplasms
Cyclic Peptides
Confocal Microscopy
Cell Line
Melanoma
Bacteriophages
Flow cytometry
Quantum Dots
Cell death
Cytotoxicity
Paclitaxel
Coulomb interactions
Semiconductor quantum dots
glucose-regulated proteins
Static Electricity
Microscopic examination
Screening

ASJC Scopus subject areas

  • Biochemistry

Cite this

Targeting heat shock proteins on cancer cells : Selection, characterization, and cell-penetrating properties of a peptidic GRP78 ligand. / Kim, Youngsoo; Lillo, Antonietta M.; Steiniger, Sebastian C J; Liu, Ying; Ballatore, Carlo; Anichini, Andrea; Mortarini, Roberta; Kaufmann, Gunnar F.; Zhou, Bin; Felding-Habermann, Brunhilde; Janda, Kim D.

In: Biochemistry, Vol. 45, No. 31, 08.08.2006, p. 9434-9444.

Research output: Contribution to journalArticle

Kim, Y, Lillo, AM, Steiniger, SCJ, Liu, Y, Ballatore, C, Anichini, A, Mortarini, R, Kaufmann, GF, Zhou, B, Felding-Habermann, B & Janda, KD 2006, 'Targeting heat shock proteins on cancer cells: Selection, characterization, and cell-penetrating properties of a peptidic GRP78 ligand', Biochemistry, vol. 45, no. 31, pp. 9434-9444. https://doi.org/10.1021/bi060264j
Kim, Youngsoo ; Lillo, Antonietta M. ; Steiniger, Sebastian C J ; Liu, Ying ; Ballatore, Carlo ; Anichini, Andrea ; Mortarini, Roberta ; Kaufmann, Gunnar F. ; Zhou, Bin ; Felding-Habermann, Brunhilde ; Janda, Kim D. / Targeting heat shock proteins on cancer cells : Selection, characterization, and cell-penetrating properties of a peptidic GRP78 ligand. In: Biochemistry. 2006 ; Vol. 45, No. 31. pp. 9434-9444.
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