TY - JOUR
T1 - Tau protein immunolocalization in fetal and adult human spinal cord
AU - Liberini, P.
AU - Valerio, A.
AU - Moretto, G.
AU - Rizzonelli, P.
AU - Memo, M.
AU - Rizzuto, N.
AU - Spano, P. F.
PY - 1995
Y1 - 1995
N2 - In the present study, the monoclonal antibody Alz-50 has been used to determine and compare the immunohistochemical localization of phosphorylated tau proteins in the developing and normal adult spinal cord. At all stages of fetal life Alz-50 fiber immunoreactivity was observed in the dorsal roots, in the dorsal and dorsolateral funiculi, and in restricted regions of the dorsal horn. Alz-50 immunoreactivity was also demonstrated in the dorsal root ganglion neurons. In the adult spinal cord a consistent pattern of Alz-50 fiber immunoreactivity was localized in the superficial layers of the dorsal horn (lamina I and II) but not in dorsal and dorsolateral funiculi and in the dorsal root ganglion. Comparable results in fetal specimens have been obtained employing PHF-1, a monoclonal antibody generated against paired helical filament proteins from Alzheimer brains, while no significant immunostaining for PHF-1 was observed in the adult spinal cord. In addition, the staining with monoclonal and polyclonal anti-tau antibodies overlapped with that of Alz-50. The transient, selective pattern of Alz-50 and PHF-1 immunoreactivity may disclose some relevant functions of tau proteins during somatosensory pathway development.
AB - In the present study, the monoclonal antibody Alz-50 has been used to determine and compare the immunohistochemical localization of phosphorylated tau proteins in the developing and normal adult spinal cord. At all stages of fetal life Alz-50 fiber immunoreactivity was observed in the dorsal roots, in the dorsal and dorsolateral funiculi, and in restricted regions of the dorsal horn. Alz-50 immunoreactivity was also demonstrated in the dorsal root ganglion neurons. In the adult spinal cord a consistent pattern of Alz-50 fiber immunoreactivity was localized in the superficial layers of the dorsal horn (lamina I and II) but not in dorsal and dorsolateral funiculi and in the dorsal root ganglion. Comparable results in fetal specimens have been obtained employing PHF-1, a monoclonal antibody generated against paired helical filament proteins from Alzheimer brains, while no significant immunostaining for PHF-1 was observed in the adult spinal cord. In addition, the staining with monoclonal and polyclonal anti-tau antibodies overlapped with that of Alz-50. The transient, selective pattern of Alz-50 and PHF-1 immunoreactivity may disclose some relevant functions of tau proteins during somatosensory pathway development.
KW - Alz-50 monoclonal antibody
KW - Development
KW - Fetus
KW - Human
KW - PHF-1 monoclonal antibody
KW - Spinal cord
KW - Tau protein
UR - http://www.scopus.com/inward/record.url?scp=0029038792&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029038792&partnerID=8YFLogxK
U2 - 10.1016/0168-0102(95)00897-2
DO - 10.1016/0168-0102(95)00897-2
M3 - Article
C2 - 7566700
AN - SCOPUS:0029038792
VL - 22
SP - 197
EP - 202
JO - Neuroscience Research
JF - Neuroscience Research
SN - 0168-0102
IS - 2
ER -