Taurine Administration Recovers Motor and Learning Deficits in an Angelman Syndrome Mouse Model

Sara Guzzetti, Luciano Calzari, Lucia Buccarello, Valentina Cesari, Ivan Toschi, Stefania Cattaldo, Alessandro Mauro, Francesca Pregnolato, Silvia Michela Mazzola, Silvia Russo

Research output: Contribution to journalArticle

Abstract

Angelman syndrome (AS, MIM 105830) is a rare neurodevelopmental disorder affecting 1:10-20,000 children. Patients show moderate to severe intellectual disability, ataxia and absence of speech. Studies on both post-mortem AS human brains and mouse models revealed dysfunctions in the extra synaptic gamma-aminobutyric acid (GABA) receptors implicated in the pathogenesis. Taurine is a free intracellular sulfur-containing amino acid, abundant in brain, considered an inhibiting neurotransmitter with neuroprotective properties. As taurine acts as an agonist of GABA-A receptors, we aimed at investigating whether it might ameliorate AS symptoms. Since mice weaning, we orally administered 1 g/kg/day taurine in water to Ube3a-deficient mice. To test the improvement of motor and cognitive skills, Rotarod, Novel Object Recognition and Open Field tests were assayed at 7, 14, 21 and 30 weeks, while biochemical tests and amino acid dosages were carried out, respectively, by Western-blot and high-performance liquid chromatography (HPLC) on frozen whole brains. Treatment of Ube3am-/p+ mice with taurine significantly improved motor and learning skills and restored the levels of the post-synaptic PSD-95 and pERK1/2-ERK1/2 ratio to wild type values. No side effects of taurine were observed. Our study indicates taurine administration as a potential therapy to ameliorate motor deficits and learning difficulties in AS.

Original languageEnglish
JournalInternational Journal of Molecular Sciences
Volume19
Issue number4
DOIs
Publication statusPublished - Apr 5 2018

Fingerprint

Angelman Syndrome
Taurine
learning
sensorimotor performance
mice
Brain
Learning
brain
Amino acids
acids
amino acids
Motor Skills
GABA Receptors
Acids
Object recognition
High performance liquid chromatography
ataxia
MIM (semiconductors)
neurotransmitters
disabilities

Keywords

  • Angelman Syndrome/drug therapy
  • Animals
  • Disease Models, Animal
  • Female
  • Learning/drug effects
  • Male
  • Mice
  • Taurine/therapeutic use
  • gamma-Aminobutyric Acid/metabolism

Cite this

Taurine Administration Recovers Motor and Learning Deficits in an Angelman Syndrome Mouse Model. / Guzzetti, Sara; Calzari, Luciano; Buccarello, Lucia; Cesari, Valentina; Toschi, Ivan; Cattaldo, Stefania; Mauro, Alessandro; Pregnolato, Francesca; Mazzola, Silvia Michela; Russo, Silvia.

In: International Journal of Molecular Sciences, Vol. 19, No. 4, 05.04.2018.

Research output: Contribution to journalArticle

Guzzetti, Sara ; Calzari, Luciano ; Buccarello, Lucia ; Cesari, Valentina ; Toschi, Ivan ; Cattaldo, Stefania ; Mauro, Alessandro ; Pregnolato, Francesca ; Mazzola, Silvia Michela ; Russo, Silvia. / Taurine Administration Recovers Motor and Learning Deficits in an Angelman Syndrome Mouse Model. In: International Journal of Molecular Sciences. 2018 ; Vol. 19, No. 4.
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AU - Toschi, Ivan

AU - Cattaldo, Stefania

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AB - Angelman syndrome (AS, MIM 105830) is a rare neurodevelopmental disorder affecting 1:10-20,000 children. Patients show moderate to severe intellectual disability, ataxia and absence of speech. Studies on both post-mortem AS human brains and mouse models revealed dysfunctions in the extra synaptic gamma-aminobutyric acid (GABA) receptors implicated in the pathogenesis. Taurine is a free intracellular sulfur-containing amino acid, abundant in brain, considered an inhibiting neurotransmitter with neuroprotective properties. As taurine acts as an agonist of GABA-A receptors, we aimed at investigating whether it might ameliorate AS symptoms. Since mice weaning, we orally administered 1 g/kg/day taurine in water to Ube3a-deficient mice. To test the improvement of motor and cognitive skills, Rotarod, Novel Object Recognition and Open Field tests were assayed at 7, 14, 21 and 30 weeks, while biochemical tests and amino acid dosages were carried out, respectively, by Western-blot and high-performance liquid chromatography (HPLC) on frozen whole brains. Treatment of Ube3am-/p+ mice with taurine significantly improved motor and learning skills and restored the levels of the post-synaptic PSD-95 and pERK1/2-ERK1/2 ratio to wild type values. No side effects of taurine were observed. Our study indicates taurine administration as a potential therapy to ameliorate motor deficits and learning difficulties in AS.

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