Taurodeoxycholate-induced intestinal injury is modulated by oxidative stress-dependent pre-conditioning like mechanisms

Piero Portincasa, Ignazio Grattagliano, Michele Petruzzelli, Antonio Moschetta, Lucantonio Debellis, Giuseppe Palasciano

Research output: Contribution to journalArticle

Abstract

Mechanisms by which hydrophobic bile salts cause tissue changes below their critical micellar concentration (CMC, 1-2 mM) and above (4-8 mM) remain poorly understood. In this study, rat colonic mucosa was exposed to different concentrations of taurodeoxycholate (TDC), t-butyl-hydroperoxide (t-BH) or glutathione ester with or without pre-incubation with 2 mM TDC. Exposure to 2 mM TDC was associated with 10% higher tissue levels of total glutathione (GSH, basal values: 33.7 ± 3.3 nmol/mg prot). With TDC 8 mM, GSH decreased to 16.4 ± 2.3 nmol/mg prot (P <0.05), oxidized glutathione (GSSG) increased by 60% (P <0.05), glutathione peroxidase (GSH-Px) and reductase activities were threefold increased, protein carbonyls fourfold increased, protein sulfhydrils decreased by 78%, lactate dehydrogenase (LDH) and GSSG release in the incubation medium were sixfold higher. In 2 mM TDC pre-treated tissues, the subsequent incubation with 8 mM TDC induced a lower loss of tissue GSH, and a lower release of LDH and GSSG. Pre-incubation with 2 mM TDC partly protected against t-BH toxicity, while glutathione ester protected against 8 mM TDC toxicity. In conclusion, TDC exposure causes opposite effects depending on CMC: induction of antioxidant protective systems including glutathione system (pre-conditioning effect) was observed with TDC below CMC, oxidative damages pointing to decreased mucosal detoxification potential with above CMC.

Original languageEnglish
Pages (from-to)36-41
Number of pages6
JournalToxicology Letters
Volume182
Issue number1-3
DOIs
Publication statusPublished - Nov 10 2008

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Taurodeoxycholic Acid
Oxidative stress
Oxidative Stress
Wounds and Injuries
Glutathione Disulfide
Glutathione
Tissue
tert-Butylhydroperoxide
L-Lactate Dehydrogenase
Toxicity
Esters
Detoxification
Glutathione Peroxidase
Bile Acids and Salts
Rats
Oxidoreductases
Mucous Membrane
Proteins
Antioxidants

Keywords

  • Bile salt toxicity
  • Glutathione
  • Lactate dehydrogenase
  • Oxidative stress
  • Protein oxidation

ASJC Scopus subject areas

  • Toxicology

Cite this

Portincasa, P., Grattagliano, I., Petruzzelli, M., Moschetta, A., Debellis, L., & Palasciano, G. (2008). Taurodeoxycholate-induced intestinal injury is modulated by oxidative stress-dependent pre-conditioning like mechanisms. Toxicology Letters, 182(1-3), 36-41. https://doi.org/10.1016/j.toxlet.2008.08.001

Taurodeoxycholate-induced intestinal injury is modulated by oxidative stress-dependent pre-conditioning like mechanisms. / Portincasa, Piero; Grattagliano, Ignazio; Petruzzelli, Michele; Moschetta, Antonio; Debellis, Lucantonio; Palasciano, Giuseppe.

In: Toxicology Letters, Vol. 182, No. 1-3, 10.11.2008, p. 36-41.

Research output: Contribution to journalArticle

Portincasa, P, Grattagliano, I, Petruzzelli, M, Moschetta, A, Debellis, L & Palasciano, G 2008, 'Taurodeoxycholate-induced intestinal injury is modulated by oxidative stress-dependent pre-conditioning like mechanisms', Toxicology Letters, vol. 182, no. 1-3, pp. 36-41. https://doi.org/10.1016/j.toxlet.2008.08.001
Portincasa, Piero ; Grattagliano, Ignazio ; Petruzzelli, Michele ; Moschetta, Antonio ; Debellis, Lucantonio ; Palasciano, Giuseppe. / Taurodeoxycholate-induced intestinal injury is modulated by oxidative stress-dependent pre-conditioning like mechanisms. In: Toxicology Letters. 2008 ; Vol. 182, No. 1-3. pp. 36-41.
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