Tax protein of human t-lymphotropic virus type I triggers DNA damage

Monica Torino, Anna Leszl, Luigi Chieco-Bianchi, Daniela Saggioro, Franca Majone, Lucia Turchetto

Research output: Contribution to journalArticlepeer-review

Abstract

Previous findings indicated that in vitro HTLV-I-infected cells are highly susceptible to spontaneous and chemically induced DNA-damage. To further study the role of different virus gene products in inducing chromosome abnormalities, MOLT-3 cells were transiently transfected with a tax expressing plasmid (pTax), and assayed for genetic damage by the micro-nucleus test. We found that pTax-transfected cells not only had a statistically higher baseline micronuclcus value than non-transfected control cells, but also were more susceptible to Mitomycin C (MMC)-induced DNA damage. Furthermore, the use of human serum containing anti-kinetochore antibodies disclosed that tax enhances the clastogenic effect of MMC. No increase in total micronuclcus frequency was observed when MMC treatment preceded pTax transfection, thus suggesting that the micronucleus increase might not be due to the additive effect of tax and MMC. These findings indicate that the viral tax protein could play an important role in inducing the chromosome damage frequently observed in HTLV-I-infected cells.

Original languageEnglish
Pages (from-to)281-286
Number of pages6
JournalLeukemia and Lymphoma
Volume12
Issue number3-4
DOIs
Publication statusPublished - 1994

Keywords

  • DNA-damage
  • HTLV-I leukemogcncsis
  • Micronuclei
  • Tax protein

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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