Tb3+ was found to bind the highest affinity Zn2+ site in alkaline phosphatase and as evidenced by the phosphorescent properties of tryptophan-109 to restore the holoconformation of the protein. Protein-bound Tb3+ displays an intense long-lived emission having two unusual features: (1) sensitization from the excited singlet state of tryptophan and (2) a lifetime (14 ms) which is five times greater than the longest-lived chelates so far known. The long-lived lanthanide emission is consistent with complete shielding of the metal ion from coordination of solvent molecules.
|Number of pages||6|
|Journal||Journal of Photochemistry and Photobiology B: Biology|
|Publication status||Published - May 15 1992|
- fluorescence lifetime
- singlet-singlet energy transfer.
- Tb luminescence
ASJC Scopus subject areas
- Plant Science
- Physical and Theoretical Chemistry