Peripheral γ/δ+ T cells were studied in patients following allogeneic bone marrow transplantation (BMT) by indirect immunofluorescence utilizing two monoclonal antibodies (G1 and A13) able to recognize the two major subpopulations (Vδ2+ and Vδ1+, respectively) of these cells. We found that the relative percentage of 'total' (γ/δ+ T lymphocytes) (Vδ2 + Vδ1 positive cells), and particularly of G1+ (Vδ2+) cells, in CD3+ lymphocytes was higher in transplanted patients, and especially in those presenting with acute graft-versus-host disease (aGVHD), than in normal controls. This finding was confirmed by the analysis of the Vδ2+/Vδ1+ cell ratio which was again significantly higher in patients with aGVHD as compared to controls. Similarly, the absolute number of 'total' γ/δ+ and Vδ2+ cells was also significantly increased in patients with aGVHD. TCR γ/δ+ T cells increased as a function of time after BMT reaching a plateau value at about day 60 post-BMT. When patients were stratified for the presence or absence of aGVHD this correlation was maintained only for patients with aGVHD. Finally, most Vδ2+ cells expressed surface T cell activation markers such as CD25 (IL-2 receptor) and DR (MHC class II) antigens. Our results suggest a possible involvement of γ/δ+ T cells and particularly of Vδ2+ cells in the clinical and immunological events (aGVHD) occurring after allogeneic BMT.
|Number of pages||5|
|Journal||Bone Marrow Transplantation|
|Publication status||Published - 1992|
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