TY - JOUR
T1 - TCTP is a critical survival factor that protects cancer cells from oxidative stress-induced cell-death
AU - Lucibello, Maria
AU - Gambacurta, Alessandra
AU - Zonfrillo, Manuela
AU - Pierimarchi, Pasquale
AU - Serafino, Annalucia
AU - Rasi, Guido
AU - Rubartelli, Anna
AU - Garaci, Enrico
PY - 2011/10/15
Y1 - 2011/10/15
N2 - The translationally controlled tumor protein (TCTP) displays growth-promoting and antiapoptotic properties. To gain information on the role of TCTP in cancer disease, we studied the modulation of TCTP and cell survival under stress conditions on tumor cell lines of different origins. When cancer cells were exposed to a mild oxidative stress, such low doses of Arsenic trioxide (ATO) or hydrogen peroxide (H
2O
2), up-regulation of TCTP was observed in cells survived to the treatment. Differently, a strong oxidative hit provided by ATO combined with glutathione (GSH) depletion or condition of glucose deprivation caused a down-modulation of TCTP followed by cell death.Clones with a forced expression of TCTP or with silenced TCTP were obtained from the breast cancer cell line MDA-MB-231. The sensitivity to oxidative stress was strongly enhanced in down-modulated TCTP cells while decreasing in cells with high levels of TCTP.Together these results indicate that TCTP is a survival factor that protects cancer cells from oxidative stress-induced cell-death. We propose TCTP as a "stress hallmark" that may be exploited as a therapeutic target to decrease the resistance of cancer cells to anticancer therapy.
AB - The translationally controlled tumor protein (TCTP) displays growth-promoting and antiapoptotic properties. To gain information on the role of TCTP in cancer disease, we studied the modulation of TCTP and cell survival under stress conditions on tumor cell lines of different origins. When cancer cells were exposed to a mild oxidative stress, such low doses of Arsenic trioxide (ATO) or hydrogen peroxide (H
2O
2), up-regulation of TCTP was observed in cells survived to the treatment. Differently, a strong oxidative hit provided by ATO combined with glutathione (GSH) depletion or condition of glucose deprivation caused a down-modulation of TCTP followed by cell death.Clones with a forced expression of TCTP or with silenced TCTP were obtained from the breast cancer cell line MDA-MB-231. The sensitivity to oxidative stress was strongly enhanced in down-modulated TCTP cells while decreasing in cells with high levels of TCTP.Together these results indicate that TCTP is a survival factor that protects cancer cells from oxidative stress-induced cell-death. We propose TCTP as a "stress hallmark" that may be exploited as a therapeutic target to decrease the resistance of cancer cells to anticancer therapy.
KW - Cancer
KW - Stress
KW - Survival
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UR - http://www.scopus.com/inward/citedby.url?scp=80052629994&partnerID=8YFLogxK
U2 - 10.1016/j.yexcr.2011.07.012
DO - 10.1016/j.yexcr.2011.07.012
M3 - Article
C2 - 21801721
AN - SCOPUS:80052629994
VL - 317
SP - 2479
EP - 2489
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 17
ER -