TE671 cell-based ELISA for anti-acetylcholine receptor antibody determination in myasthenia gravis

Diego Franciotta, Gianvito Martino, Elena Brambilla, Elisabetta Zardini, Vera Locatelli, Alessandra Bergami, Carmine Tinelli, Gaetano Desina, Vittorio Cosi

Research output: Contribution to journalArticle

Abstract

Background: Acetylcholine receptor (AChR) from human muscles is the antigen used currently in radioimmunoprecipitation assays (RIPAs) for the determination of anti-AChR antibodies in the diagnosis of myasthenia gravis (MG). Our aim was to develop and validate an ELISA using TE671 cells as the source of AChR. Methods: After TE671 cell homogenization, the crude AChR extract was used for plate coating. Anti-AChR antibodies were determined in 207 MG patients and in 77 controls. Results: The mean intra- and interassay CVs (for two samples with different anti-AChR antibody concentrations) were 9.7% and 15.7%, respectively. Test sensitivity and specificity, for generalized MG, were 79.5% (95% confidence interval, 72.8-85.0%) and 96.1% (89.0-99.1%). The detection limit was 2 nmol/L. Anti-AChR antibody concentrations from 53 MG patients, as tested with our ELISA, showed good agreement with an RIPA with a mean difference (SD) of 1.0 (5.6) nmol/L. Conclusion: Our ELISA is a simple screening test for the diagnosis of MG and enables rapid and inexpensive patient follow-up.

Original languageEnglish
Pages (from-to)400-405
Number of pages6
JournalClinical Chemistry
Volume45
Issue number3
Publication statusPublished - 1999

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Myasthenia Gravis
Cholinergic Receptors
Enzyme-Linked Immunosorbent Assay
Antibodies
Radioimmunoprecipitation Assay
Assays
Muscle
Limit of Detection
Screening
Confidence Intervals
Antigens
Sensitivity and Specificity
Muscles
Coatings

ASJC Scopus subject areas

  • Clinical Biochemistry

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TE671 cell-based ELISA for anti-acetylcholine receptor antibody determination in myasthenia gravis. / Franciotta, Diego; Martino, Gianvito; Brambilla, Elena; Zardini, Elisabetta; Locatelli, Vera; Bergami, Alessandra; Tinelli, Carmine; Desina, Gaetano; Cosi, Vittorio.

In: Clinical Chemistry, Vol. 45, No. 3, 1999, p. 400-405.

Research output: Contribution to journalArticle

Franciotta, Diego ; Martino, Gianvito ; Brambilla, Elena ; Zardini, Elisabetta ; Locatelli, Vera ; Bergami, Alessandra ; Tinelli, Carmine ; Desina, Gaetano ; Cosi, Vittorio. / TE671 cell-based ELISA for anti-acetylcholine receptor antibody determination in myasthenia gravis. In: Clinical Chemistry. 1999 ; Vol. 45, No. 3. pp. 400-405.
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AU - Martino, Gianvito

AU - Brambilla, Elena

AU - Zardini, Elisabetta

AU - Locatelli, Vera

AU - Bergami, Alessandra

AU - Tinelli, Carmine

AU - Desina, Gaetano

AU - Cosi, Vittorio

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