Tear proteomics in evaporative dry eye disease

P. Versura, P. Nanni, A. Bavelloni, W. L. Blalock, M. Piazzi, A. Roda, E. C. Campos

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: To analyze tear protein variations in patients suffering from dry eye symptoms in the presence of tear film instability but without epithelial defects. Methods: Five microlitres of non-stimulated tears from 60 patients, suffering from evaporative dry eye (EDE) with a break-up time (BUT) 10 s) were collected. Tear proteins were separated by mono and bi-dimensional SDS-PAGE electrophoresis and characterized by immunoblotting and enzymatic digestion. Digested peptides were analyzed by liquid chromatography coupled to electrospray ionization quadrupole-time of flight mass spectrometry followed by comparative data analysis into Swiss-Prot human protein database using Mascot. Statistical analysis were performed by applying a t-test for independent data and a Mann-Whitney test for unpaired data (P

Original languageEnglish
Pages (from-to)1396-1402
Number of pages7
JournalEye (London, England)
Volume24
Issue number8
DOIs
Publication statusPublished - Aug 2010

Keywords

  • dry eye disease
  • electrophoresis
  • human tears
  • mass spectrometry
  • proteomics
  • western-blot

ASJC Scopus subject areas

  • Ophthalmology

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