Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes

A study on methacarn-fixed tissue

Lorenzo Daniele, Laura Annaratone, Elena Allia, Sara Mariani, Enrico Armando, Martino Bosco, Luigia Macrì, Paola Cassoni, Giuseppe D'Armento, Gianni Bussolati, Gabor Cserni, Anna Sapino

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The optimal pathological assessment of sentinel nodes (SLNs) in breast cancer is a matter of debate. Currently, multilevel histological evaluation and immunohistochemistry (IHC) are recommended, but alternative RT-PCR procedures have been developed. To assess the reliability of these different procedures, we devised a step-sectioning protocol at 100 micron-intervals of 74 SLNs using methacarn fixation. mRNA was extracted from sections collected from levels 4 to 5. Mammaglobin, CEA and CK19 were used for RT-PCR. mRNA extraction was successful in 69 SLNs. Of these, 7 showed macrometastases (>2mm), 2 showed micrometastases (

Original languageEnglish
Pages (from-to)4042-4050
Number of pages9
JournalJournal of Cellular and Molecular Medicine
Volume13
Issue number9 B
DOIs
Publication statusPublished - Sep 2009

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Immunohistochemistry
Breast Neoplasms
Polymerase Chain Reaction
Neoplasm Micrometastasis
Messenger RNA
methacarn
cyhalothrin

Keywords

  • Breast neoplasm
  • Methacarn fixation
  • RT-PCR
  • Sentinel lymph node

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Medicine

Cite this

Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes : A study on methacarn-fixed tissue. / Daniele, Lorenzo; Annaratone, Laura; Allia, Elena; Mariani, Sara; Armando, Enrico; Bosco, Martino; Macrì, Luigia; Cassoni, Paola; D'Armento, Giuseppe; Bussolati, Gianni; Cserni, Gabor; Sapino, Anna.

In: Journal of Cellular and Molecular Medicine, Vol. 13, No. 9 B, 09.2009, p. 4042-4050.

Research output: Contribution to journalArticle

Daniele, L, Annaratone, L, Allia, E, Mariani, S, Armando, E, Bosco, M, Macrì, L, Cassoni, P, D'Armento, G, Bussolati, G, Cserni, G & Sapino, A 2009, 'Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes: A study on methacarn-fixed tissue', Journal of Cellular and Molecular Medicine, vol. 13, no. 9 B, pp. 4042-4050. https://doi.org/10.1111/j.1582-4934.2008.00449.x
Daniele, Lorenzo ; Annaratone, Laura ; Allia, Elena ; Mariani, Sara ; Armando, Enrico ; Bosco, Martino ; Macrì, Luigia ; Cassoni, Paola ; D'Armento, Giuseppe ; Bussolati, Gianni ; Cserni, Gabor ; Sapino, Anna. / Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes : A study on methacarn-fixed tissue. In: Journal of Cellular and Molecular Medicine. 2009 ; Vol. 13, No. 9 B. pp. 4042-4050.
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