Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes: A study on methacarn-fixed tissue

Lorenzo Daniele; Laura Annaratone; Elena Allia; Sara Mariani; Enrico Armando; Martino Bosco; Luigia Macrì; Paola Cassoni; Giuseppe D'Armento; Gianni Bussolati; Gabor Cserni; Anna Sapino

doi:10.1111/j.1582-4934.2008.00449.x

Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes: A study on methacarn-fixed tissue

Lorenzo Daniele, Laura Annaratone, Elena Allia, Sara Mariani, Enrico Armando, Martino Bosco, Luigia Macrì, Paola Cassoni, Giuseppe D'Armento, Gianni Bussolati, Gabor Cserni, Anna Sapino

Istituto Oncologico Candiolo

Research output: Contribution to journal › Article

Abstract

The optimal pathological assessment of sentinel nodes (SLNs) in breast cancer is a matter of debate. Currently, multilevel histological evaluation and immunohistochemistry (IHC) are recommended, but alternative RT-PCR procedures have been developed. To assess the reliability of these different procedures, we devised a step-sectioning protocol at 100 micron-intervals of 74 SLNs using methacarn fixation. mRNA was extracted from sections collected from levels 4 to 5. Mammaglobin, CEA and CK19 were used for RT-PCR. mRNA extraction was successful in 69 SLNs. Of these, 7 showed macrometastases (>2mm), 2 showed micrometastases (

Original language	English
Pages (from-to)	4042-4050
Number of pages	9
Journal	Journal of Cellular and Molecular Medicine
Volume	13
Issue number	9 B
DOIs	https://doi.org/10.1111/j.1582-4934.2008.00449.x
Publication status	Published - Sep 2009

Keywords

Breast neoplasm
Methacarn fixation
RT-PCR
Sentinel lymph node

ASJC Scopus subject areas

Cell Biology
Molecular Medicine

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Daniele, L., Annaratone, L., Allia, E., Mariani, S., Armando, E., Bosco, M., Macrì, L., Cassoni, P., D'Armento, G., Bussolati, G., Cserni, G., & Sapino, A. (2009). Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes: A study on methacarn-fixed tissue. Journal of Cellular and Molecular Medicine, 13(9 B), 4042-4050. https://doi.org/10.1111/j.1582-4934.2008.00449.x

Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes : A study on methacarn-fixed tissue. / Daniele, Lorenzo; Annaratone, Laura; Allia, Elena; Mariani, Sara; Armando, Enrico; Bosco, Martino; Macrì, Luigia; Cassoni, Paola; D'Armento, Giuseppe; Bussolati, Gianni; Cserni, Gabor; Sapino, Anna.

In: Journal of Cellular and Molecular Medicine, Vol. 13, No. 9 B, 09.2009, p. 4042-4050.

Research output: Contribution to journal › Article

Daniele, L, Annaratone, L, Allia, E, Mariani, S, Armando, E, Bosco, M, Macrì, L, Cassoni, P, D'Armento, G, Bussolati, G, Cserni, G & Sapino, A 2009, 'Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes: A study on methacarn-fixed tissue', Journal of Cellular and Molecular Medicine, vol. 13, no. 9 B, pp. 4042-4050. https://doi.org/10.1111/j.1582-4934.2008.00449.x

Daniele, Lorenzo ; Annaratone, Laura ; Allia, Elena ; Mariani, Sara ; Armando, Enrico ; Bosco, Martino ; Macrì, Luigia ; Cassoni, Paola ; D'Armento, Giuseppe ; Bussolati, Gianni ; Cserni, Gabor ; Sapino, Anna. / Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes : A study on methacarn-fixed tissue. In: Journal of Cellular and Molecular Medicine. 2009 ; Vol. 13, No. 9 B. pp. 4042-4050.

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