Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes: A study on methacarn-fixed tissue

Lorenzo Daniele, Laura Annaratone, Elena Allia, Sara Mariani, Enrico Armando, Martino Bosco, Luigia Macrì, Paola Cassoni, Giuseppe D'Armento, Gianni Bussolati, Gabor Cserni, Anna Sapino

Research output: Contribution to journalArticlepeer-review

Abstract

The optimal pathological assessment of sentinel nodes (SLNs) in breast cancer is a matter of debate. Currently, multilevel histological evaluation and immunohistochemistry (IHC) are recommended, but alternative RT-PCR procedures have been developed. To assess the reliability of these different procedures, we devised a step-sectioning protocol at 100 micron-intervals of 74 SLNs using methacarn fixation. mRNA was extracted from sections collected from levels 4 to 5. Mammaglobin, CEA and CK19 were used for RT-PCR. mRNA extraction was successful in 69 SLNs. Of these, 7 showed macrometastases (>2mm), 2 showed micrometastases (

Original languageEnglish
Pages (from-to)4042-4050
Number of pages9
JournalJournal of Cellular and Molecular Medicine
Volume13
Issue number9 B
DOIs
Publication statusPublished - Sep 2009

Keywords

  • Breast neoplasm
  • Methacarn fixation
  • RT-PCR
  • Sentinel lymph node

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Medicine

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