Abstract
The optimal pathological assessment of sentinel nodes (SLNs) in breast cancer is a matter of debate. Currently, multilevel histological evaluation and immunohistochemistry (IHC) are recommended, but alternative RT-PCR procedures have been developed. To assess the reliability of these different procedures, we devised a step-sectioning protocol at 100 micron-intervals of 74 SLNs using methacarn fixation. mRNA was extracted from sections collected from levels 4 to 5. Mammaglobin, CEA and CK19 were used for RT-PCR. mRNA extraction was successful in 69 SLNs. Of these, 7 showed macrometastases (>2mm), 2 showed micrometastases (
| Original language | English |
|---|---|
| Pages (from-to) | 4042-4050 |
| Number of pages | 9 |
| Journal | Journal of Cellular and Molecular Medicine |
| Volume | 13 |
| Issue number | 9 B |
| DOIs | |
| Publication status | Published - Sep 2009 |
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Keywords
- Breast neoplasm
- Methacarn fixation
- RT-PCR
- Sentinel lymph node
ASJC Scopus subject areas
- Cell Biology
- Molecular Medicine
Cite this
Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes : A study on methacarn-fixed tissue. / Daniele, Lorenzo; Annaratone, Laura; Allia, Elena; Mariani, Sara; Armando, Enrico; Bosco, Martino; Macrì, Luigia; Cassoni, Paola; D'Armento, Giuseppe; Bussolati, Gianni; Cserni, Gabor; Sapino, Anna.
In: Journal of Cellular and Molecular Medicine, Vol. 13, No. 9 B, 09.2009, p. 4042-4050.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes
T2 - A study on methacarn-fixed tissue
AU - Daniele, Lorenzo
AU - Annaratone, Laura
AU - Allia, Elena
AU - Mariani, Sara
AU - Armando, Enrico
AU - Bosco, Martino
AU - Macrì, Luigia
AU - Cassoni, Paola
AU - D'Armento, Giuseppe
AU - Bussolati, Gianni
AU - Cserni, Gabor
AU - Sapino, Anna
PY - 2009/9
Y1 - 2009/9
N2 - The optimal pathological assessment of sentinel nodes (SLNs) in breast cancer is a matter of debate. Currently, multilevel histological evaluation and immunohistochemistry (IHC) are recommended, but alternative RT-PCR procedures have been developed. To assess the reliability of these different procedures, we devised a step-sectioning protocol at 100 micron-intervals of 74 SLNs using methacarn fixation. mRNA was extracted from sections collected from levels 4 to 5. Mammaglobin, CEA and CK19 were used for RT-PCR. mRNA extraction was successful in 69 SLNs. Of these, 7 showed macrometastases (>2mm), 2 showed micrometastases (
AB - The optimal pathological assessment of sentinel nodes (SLNs) in breast cancer is a matter of debate. Currently, multilevel histological evaluation and immunohistochemistry (IHC) are recommended, but alternative RT-PCR procedures have been developed. To assess the reliability of these different procedures, we devised a step-sectioning protocol at 100 micron-intervals of 74 SLNs using methacarn fixation. mRNA was extracted from sections collected from levels 4 to 5. Mammaglobin, CEA and CK19 were used for RT-PCR. mRNA extraction was successful in 69 SLNs. Of these, 7 showed macrometastases (>2mm), 2 showed micrometastases (
KW - Breast neoplasm
KW - Methacarn fixation
KW - RT-PCR
KW - Sentinel lymph node
UR - http://www.scopus.com/inward/record.url?scp=77449120592&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77449120592&partnerID=8YFLogxK
U2 - 10.1111/j.1582-4934.2008.00449.x
DO - 10.1111/j.1582-4934.2008.00449.x
M3 - Article
C2 - 18671755
AN - SCOPUS:77449120592
VL - 13
SP - 4042
EP - 4050
JO - Journal of Cellular and Molecular Medicine
JF - Journal of Cellular and Molecular Medicine
SN - 1582-1838
IS - 9 B
ER -