Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes: A study on methacarn-fixed tissue

Lorenzo Daniele; Laura Annaratone; Elena Allia; Sara Mariani; Enrico Armando; Martino Bosco; Luigia Macrì; Paola Cassoni; Giuseppe D'Armento; Gianni Bussolati; Gabor Cserni; Anna Sapino

doi:10.1111/j.1582-4934.2008.00449.x

Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes: A study on methacarn-fixed tissue

Lorenzo Daniele, Laura Annaratone, Elena Allia, Sara Mariani, Enrico Armando, Martino Bosco, Luigia Macrì, Paola Cassoni, Giuseppe D'Armento, Gianni Bussolati, Gabor Cserni, Anna Sapino

Istituto Oncologico Candiolo

Research output: Contribution to journal › Article › peer-review

Abstract

The optimal pathological assessment of sentinel nodes (SLNs) in breast cancer is a matter of debate. Currently, multilevel histological evaluation and immunohistochemistry (IHC) are recommended, but alternative RT-PCR procedures have been developed. To assess the reliability of these different procedures, we devised a step-sectioning protocol at 100 micron-intervals of 74 SLNs using methacarn fixation. mRNA was extracted from sections collected from levels 4 to 5. Mammaglobin, CEA and CK19 were used for RT-PCR. mRNA extraction was successful in 69 SLNs. Of these, 7 showed macrometastases (>2mm), 2 showed micrometastases (

Original language	English
Pages (from-to)	4042-4050
Number of pages	9
Journal	Journal of Cellular and Molecular Medicine
Volume	13
Issue number	9 B
DOIs	https://doi.org/10.1111/j.1582-4934.2008.00449.x
Publication status	Published - Sep 2009

Keywords

Breast neoplasm
Methacarn fixation
RT-PCR
Sentinel lymph node

ASJC Scopus subject areas

Cell Biology
Molecular Medicine

Access to Document

10.1111/j.1582-4934.2008.00449.x

Cite this

Daniele, L., Annaratone, L., Allia, E., Mariani, S., Armando, E., Bosco, M., Macrì, L., Cassoni, P., D'Armento, G., Bussolati, G., Cserni, G., & Sapino, A. (2009). Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes: A study on methacarn-fixed tissue. Journal of Cellular and Molecular Medicine, 13(9 B), 4042-4050. https://doi.org/10.1111/j.1582-4934.2008.00449.x

Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes : A study on methacarn-fixed tissue. / Daniele, Lorenzo; Annaratone, Laura; Allia, Elena; Mariani, Sara; Armando, Enrico; Bosco, Martino; Macrì, Luigia; Cassoni, Paola; D'Armento, Giuseppe; Bussolati, Gianni; Cserni, Gabor; Sapino, Anna.

In: Journal of Cellular and Molecular Medicine, Vol. 13, No. 9 B, 09.2009, p. 4042-4050.

Research output: Contribution to journal › Article › peer-review

Daniele, L, Annaratone, L, Allia, E, Mariani, S, Armando, E, Bosco, M, Macrì, L, Cassoni, P, D'Armento, G, Bussolati, G, Cserni, G & Sapino, A 2009, 'Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes: A study on methacarn-fixed tissue', Journal of Cellular and Molecular Medicine, vol. 13, no. 9 B, pp. 4042-4050. https://doi.org/10.1111/j.1582-4934.2008.00449.x

Daniele, Lorenzo ; Annaratone, Laura ; Allia, Elena ; Mariani, Sara ; Armando, Enrico ; Bosco, Martino ; Macrì, Luigia ; Cassoni, Paola ; D'Armento, Giuseppe ; Bussolati, Gianni ; Cserni, Gabor ; Sapino, Anna. / Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes : A study on methacarn-fixed tissue. In: Journal of Cellular and Molecular Medicine. 2009 ; Vol. 13, No. 9 B. pp. 4042-4050.

@article{e624ebaa818a466ba8356b286052779e,

title = "Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes: A study on methacarn-fixed tissue",

abstract = "The optimal pathological assessment of sentinel nodes (SLNs) in breast cancer is a matter of debate. Currently, multilevel histological evaluation and immunohistochemistry (IHC) are recommended, but alternative RT-PCR procedures have been developed. To assess the reliability of these different procedures, we devised a step-sectioning protocol at 100 micron-intervals of 74 SLNs using methacarn fixation. mRNA was extracted from sections collected from levels 4 to 5. Mammaglobin, CEA and CK19 were used for RT-PCR. mRNA extraction was successful in 69 SLNs. Of these, 7 showed macrometastases (>2mm), 2 showed micrometastases (",

keywords = "Breast neoplasm, Methacarn fixation, RT-PCR, Sentinel lymph node",

author = "Lorenzo Daniele and Laura Annaratone and Elena Allia and Sara Mariani and Enrico Armando and Martino Bosco and Luigia Macr{\`i} and Paola Cassoni and Giuseppe D'Armento and Gianni Bussolati and Gabor Cserni and Anna Sapino",

year = "2009",

month = sep,

doi = "10.1111/j.1582-4934.2008.00449.x",

language = "English",

volume = "13",

pages = "4042--4050",

journal = "Journal of Cellular and Molecular Medicine",

issn = "1582-1838",

publisher = "Wiley-Blackwell",

number = "9 B",

}

TY - JOUR

T1 - Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes

T2 - A study on methacarn-fixed tissue

AU - Daniele, Lorenzo

AU - Annaratone, Laura

AU - Allia, Elena

AU - Mariani, Sara

AU - Armando, Enrico

AU - Bosco, Martino

AU - Macrì, Luigia

AU - Cassoni, Paola

AU - D'Armento, Giuseppe

AU - Bussolati, Gianni

AU - Cserni, Gabor

AU - Sapino, Anna

PY - 2009/9

Y1 - 2009/9

N2 - The optimal pathological assessment of sentinel nodes (SLNs) in breast cancer is a matter of debate. Currently, multilevel histological evaluation and immunohistochemistry (IHC) are recommended, but alternative RT-PCR procedures have been developed. To assess the reliability of these different procedures, we devised a step-sectioning protocol at 100 micron-intervals of 74 SLNs using methacarn fixation. mRNA was extracted from sections collected from levels 4 to 5. Mammaglobin, CEA and CK19 were used for RT-PCR. mRNA extraction was successful in 69 SLNs. Of these, 7 showed macrometastases (>2mm), 2 showed micrometastases (

AB - The optimal pathological assessment of sentinel nodes (SLNs) in breast cancer is a matter of debate. Currently, multilevel histological evaluation and immunohistochemistry (IHC) are recommended, but alternative RT-PCR procedures have been developed. To assess the reliability of these different procedures, we devised a step-sectioning protocol at 100 micron-intervals of 74 SLNs using methacarn fixation. mRNA was extracted from sections collected from levels 4 to 5. Mammaglobin, CEA and CK19 were used for RT-PCR. mRNA extraction was successful in 69 SLNs. Of these, 7 showed macrometastases (>2mm), 2 showed micrometastases (

KW - Breast neoplasm

KW - Methacarn fixation

KW - RT-PCR

KW - Sentinel lymph node

UR - http://www.scopus.com/inward/record.url?scp=77449120592&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77449120592&partnerID=8YFLogxK

U2 - 10.1111/j.1582-4934.2008.00449.x

DO - 10.1111/j.1582-4934.2008.00449.x

M3 - Article

C2 - 18671755

AN - SCOPUS:77449120592

VL - 13

SP - 4042

EP - 4050

JO - Journal of Cellular and Molecular Medicine

JF - Journal of Cellular and Molecular Medicine

SN - 1582-1838

IS - 9 B

ER -

Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes: A study on methacarn-fixed tissue

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this