Telomerase activity in chronic lymphoproliferative disorders of B-cell lineage

Livio Trentin, Gianna Ballon, Lucia Ometto, Alessandra Perin, Umberto Basso, Luigi Chieco-Bianchi, Gianpietro Semenzato, Anita De Rossi

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The progressive shortening of telomeres at each cell division is a key mechanism in controlling cell proliferative capacity. The activation of telomerase, a reverse transcriptase that extends telomere length, potentially leads to unlimited cell proliferation, and is believed to play a critical role in the neoplastic process. High levels of telomerase activity have been demonstrated in almost all solid tumours; however, little data is available concerning its expression in chronic B-cell neoplasms. By using a quantitative polymerase chain reaction-based method we quantified telomerase activity in normal B lymphocytes, and in various B-cell malignancies, including chronic lymphocytic leukaemia (CLL), mantle cell lymphoma (MCL) and hairy cell leukaemia (HCL). Compared to normal B cells, which expressed very low levels of telomerase activity, malignant cells from most of the patients showed a significant increase in telomerase activity, with highest values observed in HCL samples. Moreover, among the CLL and HCL cases, significantly higher levels of telomerase activity were found in patients with progressive disease at 1 year follow-up versus patients with stable disease. These data suggest that telomerase activity might correlate with disease progression.

Original languageEnglish
Pages (from-to)662-668
Number of pages7
JournalBritish Journal of Haematology
Volume106
Issue number3
DOIs
Publication statusPublished - 1999

Fingerprint

Lymphoproliferative Disorders
Telomerase
Cell Lineage
B-Lymphocytes
Hairy Cell Leukemia
B-Cell Chronic Lymphocytic Leukemia
Telomere Shortening
Neoplastic Processes
Mantle-Cell Lymphoma
Neoplasms
Telomere
Cell Division
Disease Progression
Cell Proliferation
Polymerase Chain Reaction

Keywords

  • Chronic lymphoproliferative disorders
  • Telomerase activity

ASJC Scopus subject areas

  • Hematology

Cite this

Telomerase activity in chronic lymphoproliferative disorders of B-cell lineage. / Trentin, Livio; Ballon, Gianna; Ometto, Lucia; Perin, Alessandra; Basso, Umberto; Chieco-Bianchi, Luigi; Semenzato, Gianpietro; De Rossi, Anita.

In: British Journal of Haematology, Vol. 106, No. 3, 1999, p. 662-668.

Research output: Contribution to journalArticle

Trentin, Livio ; Ballon, Gianna ; Ometto, Lucia ; Perin, Alessandra ; Basso, Umberto ; Chieco-Bianchi, Luigi ; Semenzato, Gianpietro ; De Rossi, Anita. / Telomerase activity in chronic lymphoproliferative disorders of B-cell lineage. In: British Journal of Haematology. 1999 ; Vol. 106, No. 3. pp. 662-668.
@article{12e9214c340c4d7e859f9d224ea335b7,
title = "Telomerase activity in chronic lymphoproliferative disorders of B-cell lineage",
abstract = "The progressive shortening of telomeres at each cell division is a key mechanism in controlling cell proliferative capacity. The activation of telomerase, a reverse transcriptase that extends telomere length, potentially leads to unlimited cell proliferation, and is believed to play a critical role in the neoplastic process. High levels of telomerase activity have been demonstrated in almost all solid tumours; however, little data is available concerning its expression in chronic B-cell neoplasms. By using a quantitative polymerase chain reaction-based method we quantified telomerase activity in normal B lymphocytes, and in various B-cell malignancies, including chronic lymphocytic leukaemia (CLL), mantle cell lymphoma (MCL) and hairy cell leukaemia (HCL). Compared to normal B cells, which expressed very low levels of telomerase activity, malignant cells from most of the patients showed a significant increase in telomerase activity, with highest values observed in HCL samples. Moreover, among the CLL and HCL cases, significantly higher levels of telomerase activity were found in patients with progressive disease at 1 year follow-up versus patients with stable disease. These data suggest that telomerase activity might correlate with disease progression.",
keywords = "Chronic lymphoproliferative disorders, Telomerase activity",
author = "Livio Trentin and Gianna Ballon and Lucia Ometto and Alessandra Perin and Umberto Basso and Luigi Chieco-Bianchi and Gianpietro Semenzato and {De Rossi}, Anita",
year = "1999",
doi = "10.1046/j.1365-2141.1999.01620.x",
language = "English",
volume = "106",
pages = "662--668",
journal = "British Journal of Haematology",
issn = "0007-1048",
publisher = "John Wiley & Sons, Ltd (10.1111)",
number = "3",

}

TY - JOUR

T1 - Telomerase activity in chronic lymphoproliferative disorders of B-cell lineage

AU - Trentin, Livio

AU - Ballon, Gianna

AU - Ometto, Lucia

AU - Perin, Alessandra

AU - Basso, Umberto

AU - Chieco-Bianchi, Luigi

AU - Semenzato, Gianpietro

AU - De Rossi, Anita

PY - 1999

Y1 - 1999

N2 - The progressive shortening of telomeres at each cell division is a key mechanism in controlling cell proliferative capacity. The activation of telomerase, a reverse transcriptase that extends telomere length, potentially leads to unlimited cell proliferation, and is believed to play a critical role in the neoplastic process. High levels of telomerase activity have been demonstrated in almost all solid tumours; however, little data is available concerning its expression in chronic B-cell neoplasms. By using a quantitative polymerase chain reaction-based method we quantified telomerase activity in normal B lymphocytes, and in various B-cell malignancies, including chronic lymphocytic leukaemia (CLL), mantle cell lymphoma (MCL) and hairy cell leukaemia (HCL). Compared to normal B cells, which expressed very low levels of telomerase activity, malignant cells from most of the patients showed a significant increase in telomerase activity, with highest values observed in HCL samples. Moreover, among the CLL and HCL cases, significantly higher levels of telomerase activity were found in patients with progressive disease at 1 year follow-up versus patients with stable disease. These data suggest that telomerase activity might correlate with disease progression.

AB - The progressive shortening of telomeres at each cell division is a key mechanism in controlling cell proliferative capacity. The activation of telomerase, a reverse transcriptase that extends telomere length, potentially leads to unlimited cell proliferation, and is believed to play a critical role in the neoplastic process. High levels of telomerase activity have been demonstrated in almost all solid tumours; however, little data is available concerning its expression in chronic B-cell neoplasms. By using a quantitative polymerase chain reaction-based method we quantified telomerase activity in normal B lymphocytes, and in various B-cell malignancies, including chronic lymphocytic leukaemia (CLL), mantle cell lymphoma (MCL) and hairy cell leukaemia (HCL). Compared to normal B cells, which expressed very low levels of telomerase activity, malignant cells from most of the patients showed a significant increase in telomerase activity, with highest values observed in HCL samples. Moreover, among the CLL and HCL cases, significantly higher levels of telomerase activity were found in patients with progressive disease at 1 year follow-up versus patients with stable disease. These data suggest that telomerase activity might correlate with disease progression.

KW - Chronic lymphoproliferative disorders

KW - Telomerase activity

UR - http://www.scopus.com/inward/record.url?scp=0032855045&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032855045&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2141.1999.01620.x

DO - 10.1046/j.1365-2141.1999.01620.x

M3 - Article

C2 - 10468854

AN - SCOPUS:0032855045

VL - 106

SP - 662

EP - 668

JO - British Journal of Haematology

JF - British Journal of Haematology

SN - 0007-1048

IS - 3

ER -