Telomere shortening in mucosa surrounding the tumor: Biosensor of field cancerization and prognostic marker of mucosal failure in head and neck squamous cell carcinoma

Paolo Boscolo-Rizzo, Enrica Rampazzo, Egle Perissinotto, Maria Assunta Piano, Silvia Giunco, Lorena Baboci, Giacomo Spinato, Roberto Spinato, Giancarlo Tirelli, Maria Cristina Da Mosto, Annarosa Del Mistro, Anita De Rossi

Research output: Contribution to journalArticle

Abstract

Objectives: The aim of the present study was to investigate the pattern of telomere length and telomerase expression in cancer tissues and the surrounding mucosa (SM), as markers of field cancerization and clinical outcome in patients successfully treated for with head and neck squamous cell carcinoma (HNSCC). Materials and methods: This investigation was a prospective cohort study. Telomere length and levels of telomerase reverse transcriptase (TERT) transcripts were quantified by real-time PCR in cancer tissues and SM from 139 and 90 patients with HNSCC, respectively. Results: No correlation was found between age and telomere length in SM. Patients with short telomeres in SM had a higher risk of mucosal failure (adjusted HR = 4.29). Patients with high TERT levels in cancer tissues had a higher risk of regional failure (HR = 2.88), distant failure (HR= 7.27), worse disease-specific survival (HR for related death = 2.62) but not mucosal failure. High-risk patients having both short telomeres in SM and high levels of TERT in cancer showed a significantly lower overall survival (HR = 2.46). Conclusions: Overall these findings suggest that telomere shortening in SM is a marker of field cancerization and may precede reactivation of TERT. Short telomeres in SM are strongly prognostic of mucosal failure, whereas TERT levels in cancer tissues increase with the aggressiveness of the disease and are prognostic of tumor spread.

Original languageEnglish
Pages (from-to)500-507
Number of pages8
JournalOral Oncology
Volume51
Issue number5
DOIs
Publication statusPublished - 2015

Keywords

  • Field cancerization
  • Head and neck cancer
  • Human papilloma virus
  • Local relapse
  • Molecular biology
  • Survival
  • Telomerase reverse transcriptase
  • Telomeres
  • TERT

ASJC Scopus subject areas

  • Oncology
  • Oral Surgery
  • Cancer Research
  • Medicine(all)

Fingerprint Dive into the research topics of 'Telomere shortening in mucosa surrounding the tumor: Biosensor of field cancerization and prognostic marker of mucosal failure in head and neck squamous cell carcinoma'. Together they form a unique fingerprint.

  • Cite this