Telomere shortening in mucosa surrounding the tumor: Biosensor of field cancerization and prognostic marker of mucosal failure in head and neck squamous cell carcinoma

Paolo Boscolo-Rizzo, Enrica Rampazzo, Egle Perissinotto, Maria Assunta Piano, Silvia Giunco, Lorena Baboci, Giacomo Spinato, Roberto Spinato, Giancarlo Tirelli, Maria Cristina Da Mosto, Annarosa Del Mistro, Anita De Rossi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Objectives: The aim of the present study was to investigate the pattern of telomere length and telomerase expression in cancer tissues and the surrounding mucosa (SM), as markers of field cancerization and clinical outcome in patients successfully treated for with head and neck squamous cell carcinoma (HNSCC). Materials and methods: This investigation was a prospective cohort study. Telomere length and levels of telomerase reverse transcriptase (TERT) transcripts were quantified by real-time PCR in cancer tissues and SM from 139 and 90 patients with HNSCC, respectively. Results: No correlation was found between age and telomere length in SM. Patients with short telomeres in SM had a higher risk of mucosal failure (adjusted HR = 4.29). Patients with high TERT levels in cancer tissues had a higher risk of regional failure (HR = 2.88), distant failure (HR= 7.27), worse disease-specific survival (HR for related death = 2.62) but not mucosal failure. High-risk patients having both short telomeres in SM and high levels of TERT in cancer showed a significantly lower overall survival (HR = 2.46). Conclusions: Overall these findings suggest that telomere shortening in SM is a marker of field cancerization and may precede reactivation of TERT. Short telomeres in SM are strongly prognostic of mucosal failure, whereas TERT levels in cancer tissues increase with the aggressiveness of the disease and are prognostic of tumor spread.

Original languageEnglish
Pages (from-to)500-507
Number of pages8
JournalOral Oncology
Volume51
Issue number5
DOIs
Publication statusPublished - 2015

Fingerprint

Telomere Shortening
Biosensing Techniques
Telomerase
Telomere
Mucous Membrane
Neoplasms
Survival
Carcinoma, squamous cell of head and neck
Real-Time Polymerase Chain Reaction
Cohort Studies
Prospective Studies

Keywords

  • Field cancerization
  • Head and neck cancer
  • Human papilloma virus
  • Local relapse
  • Molecular biology
  • Survival
  • Telomerase reverse transcriptase
  • Telomeres
  • TERT

ASJC Scopus subject areas

  • Oncology
  • Oral Surgery
  • Cancer Research
  • Medicine(all)

Cite this

Telomere shortening in mucosa surrounding the tumor : Biosensor of field cancerization and prognostic marker of mucosal failure in head and neck squamous cell carcinoma. / Boscolo-Rizzo, Paolo; Rampazzo, Enrica; Perissinotto, Egle; Piano, Maria Assunta; Giunco, Silvia; Baboci, Lorena; Spinato, Giacomo; Spinato, Roberto; Tirelli, Giancarlo; Da Mosto, Maria Cristina; Del Mistro, Annarosa; De Rossi, Anita.

In: Oral Oncology, Vol. 51, No. 5, 2015, p. 500-507.

Research output: Contribution to journalArticle

Boscolo-Rizzo, Paolo ; Rampazzo, Enrica ; Perissinotto, Egle ; Piano, Maria Assunta ; Giunco, Silvia ; Baboci, Lorena ; Spinato, Giacomo ; Spinato, Roberto ; Tirelli, Giancarlo ; Da Mosto, Maria Cristina ; Del Mistro, Annarosa ; De Rossi, Anita. / Telomere shortening in mucosa surrounding the tumor : Biosensor of field cancerization and prognostic marker of mucosal failure in head and neck squamous cell carcinoma. In: Oral Oncology. 2015 ; Vol. 51, No. 5. pp. 500-507.
@article{cfea5109d3734cc4ba3f13ae2efb2f7e,
title = "Telomere shortening in mucosa surrounding the tumor: Biosensor of field cancerization and prognostic marker of mucosal failure in head and neck squamous cell carcinoma",
abstract = "Objectives: The aim of the present study was to investigate the pattern of telomere length and telomerase expression in cancer tissues and the surrounding mucosa (SM), as markers of field cancerization and clinical outcome in patients successfully treated for with head and neck squamous cell carcinoma (HNSCC). Materials and methods: This investigation was a prospective cohort study. Telomere length and levels of telomerase reverse transcriptase (TERT) transcripts were quantified by real-time PCR in cancer tissues and SM from 139 and 90 patients with HNSCC, respectively. Results: No correlation was found between age and telomere length in SM. Patients with short telomeres in SM had a higher risk of mucosal failure (adjusted HR = 4.29). Patients with high TERT levels in cancer tissues had a higher risk of regional failure (HR = 2.88), distant failure (HR= 7.27), worse disease-specific survival (HR for related death = 2.62) but not mucosal failure. High-risk patients having both short telomeres in SM and high levels of TERT in cancer showed a significantly lower overall survival (HR = 2.46). Conclusions: Overall these findings suggest that telomere shortening in SM is a marker of field cancerization and may precede reactivation of TERT. Short telomeres in SM are strongly prognostic of mucosal failure, whereas TERT levels in cancer tissues increase with the aggressiveness of the disease and are prognostic of tumor spread.",
keywords = "Field cancerization, Head and neck cancer, Human papilloma virus, Local relapse, Molecular biology, Survival, Telomerase reverse transcriptase, Telomeres, TERT",
author = "Paolo Boscolo-Rizzo and Enrica Rampazzo and Egle Perissinotto and Piano, {Maria Assunta} and Silvia Giunco and Lorena Baboci and Giacomo Spinato and Roberto Spinato and Giancarlo Tirelli and {Da Mosto}, {Maria Cristina} and {Del Mistro}, Annarosa and {De Rossi}, Anita",
year = "2015",
doi = "10.1016/j.oraloncology.2015.02.100",
language = "English",
volume = "51",
pages = "500--507",
journal = "Oral Oncology",
issn = "1368-8375",
publisher = "Elsevier Limited",
number = "5",

}

TY - JOUR

T1 - Telomere shortening in mucosa surrounding the tumor

T2 - Biosensor of field cancerization and prognostic marker of mucosal failure in head and neck squamous cell carcinoma

AU - Boscolo-Rizzo, Paolo

AU - Rampazzo, Enrica

AU - Perissinotto, Egle

AU - Piano, Maria Assunta

AU - Giunco, Silvia

AU - Baboci, Lorena

AU - Spinato, Giacomo

AU - Spinato, Roberto

AU - Tirelli, Giancarlo

AU - Da Mosto, Maria Cristina

AU - Del Mistro, Annarosa

AU - De Rossi, Anita

PY - 2015

Y1 - 2015

N2 - Objectives: The aim of the present study was to investigate the pattern of telomere length and telomerase expression in cancer tissues and the surrounding mucosa (SM), as markers of field cancerization and clinical outcome in patients successfully treated for with head and neck squamous cell carcinoma (HNSCC). Materials and methods: This investigation was a prospective cohort study. Telomere length and levels of telomerase reverse transcriptase (TERT) transcripts were quantified by real-time PCR in cancer tissues and SM from 139 and 90 patients with HNSCC, respectively. Results: No correlation was found between age and telomere length in SM. Patients with short telomeres in SM had a higher risk of mucosal failure (adjusted HR = 4.29). Patients with high TERT levels in cancer tissues had a higher risk of regional failure (HR = 2.88), distant failure (HR= 7.27), worse disease-specific survival (HR for related death = 2.62) but not mucosal failure. High-risk patients having both short telomeres in SM and high levels of TERT in cancer showed a significantly lower overall survival (HR = 2.46). Conclusions: Overall these findings suggest that telomere shortening in SM is a marker of field cancerization and may precede reactivation of TERT. Short telomeres in SM are strongly prognostic of mucosal failure, whereas TERT levels in cancer tissues increase with the aggressiveness of the disease and are prognostic of tumor spread.

AB - Objectives: The aim of the present study was to investigate the pattern of telomere length and telomerase expression in cancer tissues and the surrounding mucosa (SM), as markers of field cancerization and clinical outcome in patients successfully treated for with head and neck squamous cell carcinoma (HNSCC). Materials and methods: This investigation was a prospective cohort study. Telomere length and levels of telomerase reverse transcriptase (TERT) transcripts were quantified by real-time PCR in cancer tissues and SM from 139 and 90 patients with HNSCC, respectively. Results: No correlation was found between age and telomere length in SM. Patients with short telomeres in SM had a higher risk of mucosal failure (adjusted HR = 4.29). Patients with high TERT levels in cancer tissues had a higher risk of regional failure (HR = 2.88), distant failure (HR= 7.27), worse disease-specific survival (HR for related death = 2.62) but not mucosal failure. High-risk patients having both short telomeres in SM and high levels of TERT in cancer showed a significantly lower overall survival (HR = 2.46). Conclusions: Overall these findings suggest that telomere shortening in SM is a marker of field cancerization and may precede reactivation of TERT. Short telomeres in SM are strongly prognostic of mucosal failure, whereas TERT levels in cancer tissues increase with the aggressiveness of the disease and are prognostic of tumor spread.

KW - Field cancerization

KW - Head and neck cancer

KW - Human papilloma virus

KW - Local relapse

KW - Molecular biology

KW - Survival

KW - Telomerase reverse transcriptase

KW - Telomeres

KW - TERT

UR - http://www.scopus.com/inward/record.url?scp=84933670812&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84933670812&partnerID=8YFLogxK

U2 - 10.1016/j.oraloncology.2015.02.100

DO - 10.1016/j.oraloncology.2015.02.100

M3 - Article

C2 - 25771075

AN - SCOPUS:84933670812

VL - 51

SP - 500

EP - 507

JO - Oral Oncology

JF - Oral Oncology

SN - 1368-8375

IS - 5

ER -