Telomeres increasingly develop aberrant structures in aging humans

Virginia Boccardi, Luigi Cari, Giuseppe Nocentini, Carlo Riccardi, Roberta Cecchetti, Carmelinda Ruggiero, Beatrice Arosio, Giuseppe Paolisso, Utz Herbig, Patrizia Mecocci

Research output: Contribution to journalArticle

Abstract

Telomeres progressively shorten with age, and it has been proposed that critically short and dysfunctional telomeres contribute to aging and aging-associated diseases in humans. For many years it was thought that telomere erosion was strictly a consequence of the “end replication problem,” or the inability of replicative polymerases to completely duplicate linear DNA ends. It is becoming increasingly evident, however, that telomere shortening of cultured human cells is also caused because of other replication defects in telomeric repeats, those that cause fragile telomeres and other aberrant telomeric structures that can be detected on metaphase chromosomes. Whether these replication defects contribute to telomere erosion also in human tissues is currently unknown. By analyzing peripheral blood mononuclear cells from a total of 35 healthy subjects ranging in age from 23 to 101 years, we demonstrated that telomeres increasingly display aberrant structures with advancing donor age. Although the percentages of fragile telomeres increased only until adulthood, the percentages of chromosomes displaying sister telomere loss and sister telomere chromatid fusions increased consistently throughout the entire human life span. Our data, therefore, suggest that telomeric replication defects other than the end replication problem contribute to aging-associated telomere erosion in humans.

Original languageEnglish
Pages (from-to)230-235
Number of pages6
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume75
Issue number2
DOIs
Publication statusPublished - Feb 1 2020

Keywords

  • Health
  • Human aging
  • Telomeres

ASJC Scopus subject areas

  • Ageing
  • Geriatrics and Gerontology

Fingerprint Dive into the research topics of 'Telomeres increasingly develop aberrant structures in aging humans'. Together they form a unique fingerprint.

  • Cite this

    Boccardi, V., Cari, L., Nocentini, G., Riccardi, C., Cecchetti, R., Ruggiero, C., Arosio, B., Paolisso, G., Herbig, U., & Mecocci, P. (2020). Telomeres increasingly develop aberrant structures in aging humans. Journals of Gerontology - Series A Biological Sciences and Medical Sciences, 75(2), 230-235. https://doi.org/10.1093/gerona/gly257