Telomeric DNA damage is irreparable and causes persistent DNA-damage-response activation

Marzia Fumagalli, Francesca Rossiello, Michela Clerici, Sara Barozzi, Davide Cittaro, Jessica M. Kaplunov, Gabriele Bucci, Miryana Dobreva, Valentina Matti, Christian M. Beausejour, Utz Herbig, Maria Pia Longhese, Fabrizio D Adda Di Fagagna

Research output: Contribution to journalArticle

333 Citations (Scopus)

Abstract

The DNA-damage response (DDR) arrests cell-cycle progression until damage is removed. DNA-damage-induced cellular senescence is associated with persistent DDR. The molecular bases that distinguish transient from persistent DDR are unknown. Here we show that a large fraction of exogenously induced persistent DDR markers is associated with telomeric DNA in cultured cells and mammalian tissues. In yeast, a chromosomal DNA double-strand break next to a telomeric sequence resists repair and impairs DNA ligase 4 recruitment. In mammalian cells, ectopic localization of telomeric factor TRF2 next to a double-strand break induces persistent DNA damage and DDR. Linear, but not circular, telomeric DNA or scrambled DNA induces a prolonged checkpoint in normal cells. In terminally differentiated tissues of old primates, DDR markers accumulate at telomeres that are not critically short. We propose that linear genomes are not uniformly reparable and that telomeric DNA tracts, if damaged, are irreparable and trigger persistent DDR and cellular senescence.

Original languageEnglish
Pages (from-to)355-365
Number of pages11
JournalNature Cell Biology
Volume14
Issue number4
DOIs
Publication statusPublished - Apr 2012

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DNA Damage
Cell Aging
DNA
DNA Ligases
Circular DNA
Double-Stranded DNA Breaks
Telomere
Cell Cycle Checkpoints
Primates
Cultured Cells
Yeasts
Genome

ASJC Scopus subject areas

  • Cell Biology

Cite this

Fumagalli, M., Rossiello, F., Clerici, M., Barozzi, S., Cittaro, D., Kaplunov, J. M., ... Di Fagagna, F. D. A. (2012). Telomeric DNA damage is irreparable and causes persistent DNA-damage-response activation. Nature Cell Biology, 14(4), 355-365. https://doi.org/10.1038/ncb2466

Telomeric DNA damage is irreparable and causes persistent DNA-damage-response activation. / Fumagalli, Marzia; Rossiello, Francesca; Clerici, Michela; Barozzi, Sara; Cittaro, Davide; Kaplunov, Jessica M.; Bucci, Gabriele; Dobreva, Miryana; Matti, Valentina; Beausejour, Christian M.; Herbig, Utz; Longhese, Maria Pia; Di Fagagna, Fabrizio D Adda.

In: Nature Cell Biology, Vol. 14, No. 4, 04.2012, p. 355-365.

Research output: Contribution to journalArticle

Fumagalli, M, Rossiello, F, Clerici, M, Barozzi, S, Cittaro, D, Kaplunov, JM, Bucci, G, Dobreva, M, Matti, V, Beausejour, CM, Herbig, U, Longhese, MP & Di Fagagna, FDA 2012, 'Telomeric DNA damage is irreparable and causes persistent DNA-damage-response activation', Nature Cell Biology, vol. 14, no. 4, pp. 355-365. https://doi.org/10.1038/ncb2466
Fumagalli, Marzia ; Rossiello, Francesca ; Clerici, Michela ; Barozzi, Sara ; Cittaro, Davide ; Kaplunov, Jessica M. ; Bucci, Gabriele ; Dobreva, Miryana ; Matti, Valentina ; Beausejour, Christian M. ; Herbig, Utz ; Longhese, Maria Pia ; Di Fagagna, Fabrizio D Adda. / Telomeric DNA damage is irreparable and causes persistent DNA-damage-response activation. In: Nature Cell Biology. 2012 ; Vol. 14, No. 4. pp. 355-365.
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