Temozolomide induced differentiation of K562 leukemia cells is not mediated by gene hypomethylation

M. Zucchetti, C. V. Catapano, S. Filippeschi, E. Erba, M. D'Incalci

Research output: Contribution to journalArticle

Abstract

Temozolomide (8-carbamoyl-3-methylimidazo[5,1d]-1,2,3,5-tetrazin-4-(3H)-one), an experimental antitumor agent which spontaneously decomposes to 5-(3,3-methyl-1-triazeno) imidazole-4-carboxamide, the active metabolite of the antineoplastic drug DTIC, causes erythroid differentiation of K562 leukemia cells. The increase in ε and γ globin gene expression after temozolomide treatment does not appear to be due to drug-induced hypomethylation of the genes. In other genes containing many methylated sequences such as the proto-oncogenes c-myc and C-Ha-ras, temozolomide caused no detectable change in methylation. In contrast, in the same genes 5-aza-2'-deoxycytidine induced hypomethylation. Temozolomide caused DNA alkali-labile sites and an arrest of the cell cycle in G2 phase. Ethazolastone (its 3-ethylimidazo analogue) which does not cause differentiation of K562 produced no significant DNA damage and G2 phase blockade. DNA damage rather than hypomethylation may be responsible for induction of differentiation.

Original languageEnglish
Pages (from-to)2069-2075
Number of pages7
JournalBiochemical Pharmacology
Volume38
Issue number13
DOIs
Publication statusPublished - Jul 1 1989

ASJC Scopus subject areas

  • Pharmacology

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