Temperature adaptation of glutathione S-transferase P1-1. A case for homotropic regulation of substrate binding

Anna Maria Caccuri, Giovanni Antonini, Paolo Ascenzi, Maria Nicotra, Marzia Nuccetelli, Anna Paola Mazzetti, Giorgio Federici, Mario Lo Bello, Giorgio Ricci

Research output: Contribution to journalArticle

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Abstract

Human glutathione S-transferase P1-1 (GST P1-1) is a homodimeric enzyme expressed in several organs as well as in the upper layers of epidermis, playing a role against carcinogenic and toxic compounds. A sophisticated mechanism of temperature adaptation has been developed by this enzyme. In fact, above 35 °C, glutathione (GSH) binding to GST P1-1 displays positive cooperativity, whereas negative cooperativity occurs below 25 °C. This binding mechanism minimizes changes of GSH affinity for GST P1-1 because of temperature fluctuation. This is a likely advantage for epithelial skin cells, which are naturally exposed to temperature variation and, incidentally, to carcinogenic compounds, always needing efficient detoxifying systems. As a whole, GST P1-1 represents the first enzyme which displays a temperature-dependent homotropic regulation of substrate (e.g. GSH) binding.

Original languageEnglish
Pages (from-to)19276-19280
Number of pages5
JournalJournal of Biological Chemistry
Volume274
Issue number27
DOIs
Publication statusPublished - Jul 2 1999

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Glutathione S-Transferase pi
Glutathione Transferase
Temperature
Substrates
Enzymes
Poisons
Epidermis
Glutathione
Skin
Epithelial Cells
Display devices

ASJC Scopus subject areas

  • Biochemistry

Cite this

Caccuri, A. M., Antonini, G., Ascenzi, P., Nicotra, M., Nuccetelli, M., Mazzetti, A. P., ... Ricci, G. (1999). Temperature adaptation of glutathione S-transferase P1-1. A case for homotropic regulation of substrate binding. Journal of Biological Chemistry, 274(27), 19276-19280. https://doi.org/10.1074/jbc.274.27.19276

Temperature adaptation of glutathione S-transferase P1-1. A case for homotropic regulation of substrate binding. / Caccuri, Anna Maria; Antonini, Giovanni; Ascenzi, Paolo; Nicotra, Maria; Nuccetelli, Marzia; Mazzetti, Anna Paola; Federici, Giorgio; Lo Bello, Mario; Ricci, Giorgio.

In: Journal of Biological Chemistry, Vol. 274, No. 27, 02.07.1999, p. 19276-19280.

Research output: Contribution to journalArticle

Caccuri, AM, Antonini, G, Ascenzi, P, Nicotra, M, Nuccetelli, M, Mazzetti, AP, Federici, G, Lo Bello, M & Ricci, G 1999, 'Temperature adaptation of glutathione S-transferase P1-1. A case for homotropic regulation of substrate binding', Journal of Biological Chemistry, vol. 274, no. 27, pp. 19276-19280. https://doi.org/10.1074/jbc.274.27.19276
Caccuri, Anna Maria ; Antonini, Giovanni ; Ascenzi, Paolo ; Nicotra, Maria ; Nuccetelli, Marzia ; Mazzetti, Anna Paola ; Federici, Giorgio ; Lo Bello, Mario ; Ricci, Giorgio. / Temperature adaptation of glutathione S-transferase P1-1. A case for homotropic regulation of substrate binding. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 27. pp. 19276-19280.
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