TY - JOUR
T1 - Temperature-dependent protein folding in vivo - Lower growth temperature increases yield of two genetic variants of Xenopus laevis Cu, Zn superoxide dismutase in Escherichia coli
AU - Andrea Battistoni, M.
AU - Teresa Carri, A.
AU - Mazzetti, Paola
AU - Rotilio, Giuseppe
PY - 1992/8/14
Y1 - 1992/8/14
N2 - Two genetic variants of Xenopus laevis Cu,Zn superoxide dismutase, XSODA and XSODB, have been expressed in Escherichia coli by recombinant DNA techniques. Production of both proteins was obtained, although with different yields, XSODB being more abundant than XSODA in all the conditions tested. Lowering the temperature of growth was found to be a specific factor, decisive in obtaining quantitatively abundant, active Xenopus enzymes. Impaired folding of these proteins in the E. coli cytoplasm was found to parallel their in vitro properties.
AB - Two genetic variants of Xenopus laevis Cu,Zn superoxide dismutase, XSODA and XSODB, have been expressed in Escherichia coli by recombinant DNA techniques. Production of both proteins was obtained, although with different yields, XSODB being more abundant than XSODA in all the conditions tested. Lowering the temperature of growth was found to be a specific factor, decisive in obtaining quantitatively abundant, active Xenopus enzymes. Impaired folding of these proteins in the E. coli cytoplasm was found to parallel their in vitro properties.
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U2 - 10.1016/S0006-291X(05)81553-0
DO - 10.1016/S0006-291X(05)81553-0
M3 - Article
C2 - 1510665
AN - SCOPUS:0026975772
VL - 186
SP - 1339
EP - 1344
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -