Temperature-dependent protein folding in vivo - Lower growth temperature increases yield of two genetic variants of Xenopus laevis Cu, Zn superoxide dismutase in Escherichia coli

M. Andrea Battistoni; A. Teresa Carri; Paola Mazzetti; Giuseppe Rotilio

doi:10.1016/S0006-291X(05)81553-0

Temperature-dependent protein folding in vivo - Lower growth temperature increases yield of two genetic variants of Xenopus laevis Cu, Zn superoxide dismutase in Escherichia coli

M. Andrea Battistoni, A. Teresa Carri, Paola Mazzetti, Giuseppe Rotilio

Fondazione Santa Lucia

Research output: Contribution to journal › Article

Abstract

Two genetic variants of Xenopus laevis Cu,Zn superoxide dismutase, XSODA and XSODB, have been expressed in Escherichia coli by recombinant DNA techniques. Production of both proteins was obtained, although with different yields, XSODB being more abundant than XSODA in all the conditions tested. Lowering the temperature of growth was found to be a specific factor, decisive in obtaining quantitatively abundant, active Xenopus enzymes. Impaired folding of these proteins in the E. coli cytoplasm was found to parallel their in vitro properties.

Original language	English
Pages (from-to)	1339-1344
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	186
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(05)81553-0
Publication status	Published - Aug 14 1992

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ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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Andrea Battistoni, M., Teresa Carri, A., Mazzetti, P., & Rotilio, G. (1992). Temperature-dependent protein folding in vivo - Lower growth temperature increases yield of two genetic variants of Xenopus laevis Cu, Zn superoxide dismutase in Escherichia coli. Biochemical and Biophysical Research Communications, 186(3), 1339-1344. https://doi.org/10.1016/S0006-291X(05)81553-0

Temperature-dependent protein folding in vivo - Lower growth temperature increases yield of two genetic variants of Xenopus laevis Cu, Zn superoxide dismutase in Escherichia coli. / Andrea Battistoni, M.; Teresa Carri, A.; Mazzetti, Paola; Rotilio, Giuseppe.

In: Biochemical and Biophysical Research Communications, Vol. 186, No. 3, 14.08.1992, p. 1339-1344.

Research output: Contribution to journal › Article

Andrea Battistoni, M, Teresa Carri, A, Mazzetti, P & Rotilio, G 1992, 'Temperature-dependent protein folding in vivo - Lower growth temperature increases yield of two genetic variants of Xenopus laevis Cu, Zn superoxide dismutase in Escherichia coli', Biochemical and Biophysical Research Communications, vol. 186, no. 3, pp. 1339-1344. https://doi.org/10.1016/S0006-291X(05)81553-0

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10.1016/S0006-291X(05)81553-0