Temporal and tissue-specific expression of the MET ORF driven by the complete transcriptional unit of human A1AT gene in transgenic mice

Laura Amicone, Michele Alessandro Galimi, Francesca Maria Spagnoli, Cristina Tommasini, Veronica De Luca, Marco Tripodi

Research output: Contribution to journalArticlepeer-review

Abstract

We inserted the sequence coding for the cytoplasmic portion of the human MET receptor into an 18-kb genomic fragment containing the entire human A1AT gene (encoding α-l-antitrypsin). Stringent control of gene expression, at the transcriptional, post-transcriptional and translational levels, was ensured by insertion of the MET open reading frame into A1AT, thus maintaining: (i) all the elements that confer tissue-specific transcription initiation, (ii) all the sequences involved in transcript processing and (iii) all the sequences which influence messenger stability and translational efficiency. The expression pattern of this vector in transgenic mice was identical to that of the human A1AT transgene, as well as to that of A1AT in humans with regard to both temporal and tissue-specific regulation.

Original languageEnglish
Pages (from-to)323-328
Number of pages6
JournalGene
Volume162
Issue number2
Publication statusPublished - Sep 11 1995

Keywords

  • α-l-antitrypsin
  • A1AT
  • base pair(s)
  • bp
  • cytoplasmic portion of MET receptor
  • d.p.c.
  • DAB
  • days post coitum
  • diaminobenzidine
  • expression vector
  • gene encoding A1AT
  • gene encoding MET
  • Gene expression control
  • hepatocyte growth factor receptor
  • homologous recombination procedure of cloning
  • kb
  • kilobase(s) or 1000 bp
  • MET
  • MET-CYT
  • nt
  • nucleotide(s)
  • open reading frame
  • ORF
  • P
  • PCR
  • polymerase chain reaction
  • promoter
  • re-
  • recombinant
  • site-directed mutagenesis
  • untranslated region(s)
  • UTR
  • wild type
  • wt

ASJC Scopus subject areas

  • Genetics

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