Temporal coincidence between synaptic vesicle fusion and quantal secretion of acetylcholine

F. Torri-Tarelli, F. Grohovaz, R. Fesce, B. Ceccarelli

Research output: Contribution to journalArticlepeer-review

Abstract

We applied the quick-freezing technique to investigate the precise temporal coincidence between the onset of quantal secretion and the appearance of fusions of synaptic vesicles with the prejunctional membrane. Frog cutaneous pectoris nerve-muscle preparations were soaked in modified Ringer's solution with 1 mM 4-aminopyridine, 10 mM Ca2+, and 10-4 M d-Tubocurarine and quick-frozen 1-10 ms after a single supramaximal shock. The frozen muscles were then either freeze-fractured or cryosubstituted in acetone with 13% OsO4 and processed for thin section electron microscopy. Temporal resolution of

Original languageEnglish
Pages (from-to)1386-1399
Number of pages14
JournalJournal of Cell Biology
Volume101
Issue number4
Publication statusPublished - 1985

ASJC Scopus subject areas

  • Cell Biology

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