Temporin A as a prophylactic agent against methicillin sodium-susceptible and methicillin sodium-resistant Staphylococcus epidermidis vascular graft infection

Roberto Ghiselli, Andrea Giacometti, Oscar Cirioni, Federico Mocchegiani, Fiorenza Orlando, Wojciech Kamysz, Maria Simona Del Prete, Jerzy Lukasiak, Giorgio Scalise, Vittorio Saba

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Objective: The purpose of this study was to investigate the efficacy of temporin A as a prophylactic agent in a rat model of vascular graft infection from methicillin sodium-susceptible and methicillin sodium-resistant Staphylococcus epidermidis. Methods: The prospective, randomized, controlled animal study set in a research laboratory in a university hospital used 280 adult male Wistar rats (weight range, 280 to 350 g). Graft infections were established in the back subcutaneous tissue of rats with implantation of 1-cm 2 sterile Dacron grafts followed by topical inoculation with 2 × 107 colony-forming units of S epidermidis. The study for each staphylococcal strain included: one control group (no graft contamination), one contaminated group that did not receive any antibiotic prophylaxis, one contaminated group that received temporin A-soaked graft, two contaminated groups that received perioperative intraperitoneal cefazolin (30 mg/kg) or vancomycin hydrochloride prophylaxis (10 mg/kg), and two contaminated groups that received temporin A-soaked graft and perioperative intraperitoneal cefazolin (30 mg/kg) or vancomycin hydrochloride (10 mg/kg) prophylaxis. All grafts were explanted at 7 days after implantation. The main outcome measure was quantification of bacterial contamination. Results: Overall, the perioperative prophylaxis based on soaked grafts was not significantly different to that of parenteral vancomycin hydrochloride. Only the combination between temporin A and vancomycin hydrochloride produced a complete bacterial inhibition for both strains. Conclusion: Temporin A showed a similar antibacterial in vitro activity against the two different strains. The in vivo results suggest its potential use in providing prophylaxis to direct graft contamination when used in combination with parenteral vancomycin hydrochloride.

Original languageEnglish
Pages (from-to)1027-1030
Number of pages4
JournalJournal of Vascular Surgery
Volume36
Issue number5
Publication statusPublished - Nov 1 2002

Fingerprint

Methicillin Resistance
Methicillin
Staphylococcus epidermidis
Blood Vessels
Transplants
Vancomycin
Infection
Cefazolin
temporin
Polyethylene Terephthalates
Antibiotic Prophylaxis
Subcutaneous Tissue
Wistar Rats
Stem Cells
Outcome Assessment (Health Care)
Weights and Measures
Control Groups

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Temporin A as a prophylactic agent against methicillin sodium-susceptible and methicillin sodium-resistant Staphylococcus epidermidis vascular graft infection. / Ghiselli, Roberto; Giacometti, Andrea; Cirioni, Oscar; Mocchegiani, Federico; Orlando, Fiorenza; Kamysz, Wojciech; Del Prete, Maria Simona; Lukasiak, Jerzy; Scalise, Giorgio; Saba, Vittorio.

In: Journal of Vascular Surgery, Vol. 36, No. 5, 01.11.2002, p. 1027-1030.

Research output: Contribution to journalArticle

Ghiselli, R, Giacometti, A, Cirioni, O, Mocchegiani, F, Orlando, F, Kamysz, W, Del Prete, MS, Lukasiak, J, Scalise, G & Saba, V 2002, 'Temporin A as a prophylactic agent against methicillin sodium-susceptible and methicillin sodium-resistant Staphylococcus epidermidis vascular graft infection', Journal of Vascular Surgery, vol. 36, no. 5, pp. 1027-1030.
Ghiselli, Roberto ; Giacometti, Andrea ; Cirioni, Oscar ; Mocchegiani, Federico ; Orlando, Fiorenza ; Kamysz, Wojciech ; Del Prete, Maria Simona ; Lukasiak, Jerzy ; Scalise, Giorgio ; Saba, Vittorio. / Temporin A as a prophylactic agent against methicillin sodium-susceptible and methicillin sodium-resistant Staphylococcus epidermidis vascular graft infection. In: Journal of Vascular Surgery. 2002 ; Vol. 36, No. 5. pp. 1027-1030.
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AU - Orlando, Fiorenza

AU - Kamysz, Wojciech

AU - Del Prete, Maria Simona

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