Temporin A is effective in MRSA-infected wounds through bactericidal activity and acceleration of wound repair in a murine model

Oriana Simonetti, Oscar Cirioni, Gaia Goteri, Roberto Ghiselli, Wojciech Kamysz, Elzbieta Kamysz, Carmela Silvestri, Fiorenza Orlando, Claudia Barucca, Alessandro Scalise, Vittorio Saba, Giorgio Scalise, Andrea Giacometti, Annamaria Offidani

Research output: Contribution to journalArticlepeer-review


We investigated the effect of topical temporin A in the management of methicillin-resistant strain of Staphylococcus aureus (MRSA)-infected experimental surgical wounds in mice. The wound, cut through the panniculus carnosus of BALB/c mice, was inoculated with 5 × 107 colony-forming units of MRSA. Mice were treated with Allevyn, temporin A-soaked Allevyn, Allevyn and daily intraperitoneal teicoplanin (7 mg/kg), temporin A-soaked Allevyn and daily intraperitoneal teicoplanin. Main outcome measurements were: quantitative bacterial culture, histological examination with assessment of micro-vessel density and of vascular endothelial growth factor (VEGF) expression in tissue sections, and VEGF plasma levels alike. Treatment with temporin-A associated with teicoplanin injection significantly reduced bacterial load to 0.85 × 101 ± 0.1 × 101 CFU/ml. Histological examination showed that infected mice receiving temporin A-soaked Allevyn (with or without teicoplanin) had a higher degree of granulation tissue formation and collagen deposition compared to the other treated groups. A significant increase in serum VEGF expression was observed in mice receiving temporin A topically and temporin A topically associated with intraperitoneal teicoplanin. In conclusion our results demonstrated that temporin A is effective in the management of infected wounds, by a significant bacterial growth inhibition and acceleration of wound repair process.

Original languageEnglish
Pages (from-to)520-528
Number of pages9
Issue number4
Publication statusPublished - Apr 2008


  • MRSA infection
  • Teicoplanin
  • Temporin A
  • VEGF
  • Wound repair

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience


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