TY - JOUR
T1 - Terminal-restriction fragment length polymorphism analysis of biphenyl dioxygenase genes from a polychlorinated biphenyl-polluted soil
AU - Capodicasa, Serena
AU - Fedi, Stefano
AU - Carnevali, Monica
AU - Caporali, Leonardo
AU - Viti, Carlo
AU - Fava, Fabio
AU - Zannoni, Davide
PY - 2009/12
Y1 - 2009/12
N2 - Polychlorinated biphenyls (PCBs) are ubiquitous persistent organic pollutants that can be co-metabolically biotransformed by biphenyl-utilizing bacteria. In this study, terminal-restriction fragment length polymorphism (T-RFLP) was applied to the substrate specificity-determining region of the 2,3-biphenyl dioxygenase encoding genes of a microbial community found in a PCB-polluted soil. Notably, both the total biphenyl/PCB-utilizing community and its members actively expressing the 2,3-biphenyl dioxygenase gene were analyzed. T-RFLP fingerprinting along with gene library construction allowed us not only to detect biphenyl dioxygenases related to the well characterized catabolic patterns of Pseudomonas pseudoalcaligenes KF707 and Burkholderia xenovorans LB400, but also to reveal novel environmental enzyme classes displaying amino acid substitutions that may be related to broader specificity and improved catalytic properties. Furthermore, space and time of sampling along with bioavailability conditions of different PCBs were considered possible sources of profile variability.
AB - Polychlorinated biphenyls (PCBs) are ubiquitous persistent organic pollutants that can be co-metabolically biotransformed by biphenyl-utilizing bacteria. In this study, terminal-restriction fragment length polymorphism (T-RFLP) was applied to the substrate specificity-determining region of the 2,3-biphenyl dioxygenase encoding genes of a microbial community found in a PCB-polluted soil. Notably, both the total biphenyl/PCB-utilizing community and its members actively expressing the 2,3-biphenyl dioxygenase gene were analyzed. T-RFLP fingerprinting along with gene library construction allowed us not only to detect biphenyl dioxygenases related to the well characterized catabolic patterns of Pseudomonas pseudoalcaligenes KF707 and Burkholderia xenovorans LB400, but also to reveal novel environmental enzyme classes displaying amino acid substitutions that may be related to broader specificity and improved catalytic properties. Furthermore, space and time of sampling along with bioavailability conditions of different PCBs were considered possible sources of profile variability.
KW - Biphenyl dioxygenase
KW - Burkholderia xenovorans LB400
KW - PCB-Polluted soil
KW - Pseudomonas pseudoalcaligenes KF707
KW - T-RFLP analysis
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U2 - 10.1016/j.resmic.2009.10.001
DO - 10.1016/j.resmic.2009.10.001
M3 - Article
C2 - 19835952
AN - SCOPUS:70849099064
VL - 160
SP - 742
EP - 750
JO - Research in Microbiology
JF - Research in Microbiology
SN - 0923-2508
IS - 10
ER -