Testing Aβ toxicity on primary CNS cultures using drug-screening microfluidic chips

A. Ruiz, P. Joshi, R. Mastrangelo, M. Francolini, C. Verderio, M. Matteoli

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Open microscale cultures of primary central nervous system (CNS) cells have been implemented in microfluidic chips that can expose the cells to physiological fluidic shear stress conditions. Cells in the chips were exposed to differently aggregated forms of beta-amyloid (Aβ), i.e. conditions mimicking an Alzheimer's Disease environment, and treated with CNS drugs in order to assess the contribution of glial cells during pharmacological treatments. FTY720, a drug approved for the treatment of Multiple Sclerosis, was found to play a marked neuroprotective role in neuronal cultures as well as in microglia-enriched neuronal cultures, preventing neurodegeneration after cell exposure to neurotoxic oligomers of Aβ. This journal is

Original languageEnglish
Pages (from-to)2860-2866
Number of pages7
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume14
Issue number15
DOIs
Publication statusPublished - Aug 7 2014

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Central Nervous System Agents
Preclinical Drug Evaluations
Microfluidics
Neurology
Fluidics
Oligomers
Amyloid
Toxicity
Shear stress
Screening
Central Nervous System
Testing
Pharmaceutical Preparations
Microglia
Neuroglia
Multiple Sclerosis
Alzheimer Disease
Pharmacology
Fingolimod Hydrochloride

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine(all)

Cite this

Testing Aβ toxicity on primary CNS cultures using drug-screening microfluidic chips. / Ruiz, A.; Joshi, P.; Mastrangelo, R.; Francolini, M.; Verderio, C.; Matteoli, M.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 14, No. 15, 07.08.2014, p. 2860-2866.

Research output: Contribution to journalArticle

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