TY - JOUR
T1 - Testing for irritation with a multifactorial approach
T2 - Comparison of eight non-invasive measuring techniques on five different irritation types
AU - Fluhr, J. W.
AU - Kuss, O.
AU - Diepgen, T.
AU - Lazzerini, S.
AU - Pelosi, A.
AU - Gloor, M.
AU - Berardesca, E.
PY - 2001
Y1 - 2001
N2 - Background: Non-invasive bioengineering methods are widely used in the assessment of irritant skin reactions. Objectives: To assess the ability of eight non-invasive measurement techniques to distinguish changes in skin conditions over time, these changes being induced by five different irritants. Methods: The following techniques were compared in a multivariate analysis: laser-Doppler perfusion imaging (LDI), laser-Doppler flowmetry (LDF), transepidermal water loss (TEWL), visual scoring (VS), colorimetric measurements (Chromameter CR 200 a* and L* scales), Mexameter Hb scale (Mexa Hb) and capacitance (Corneometer CM 820). Irritants tested were sodium lauryl sulphate 2% (SLS), tape stripping (TS), tretinoin 0.05% (TRET), ultraviolet (UV) exposure to 30 W m-2 UVB/95 W m-2 UVA, and dithranol 0.5% (DIT). Measurements were performed at baseline and after 24, 48 and 72 h. The study was conducted on the upper back of 11 healthy volunteers of both sexes aged 27-51 years. Results: For DIT it was possible to discriminate over time with CR 200 a* and L*, VS, LDI, LDF and Mexa Hb. In SLS discrimination over time was seen with TEWL and LDF. Discrimination in TS was demonstrated for TEWL, VS, CR 200 a*, CM 820, LDF, LDI and Mexa Hb. In TRET discrimination ability was seen for LDI, LDF, Mexa Hb and VS. For UV it was possible to discriminate using VS, TEWL, LDF, LDI and Mexa Hb. Conclusions: Different irritation patterns need different measurement modalities in order to give optimal discrimination over time.
AB - Background: Non-invasive bioengineering methods are widely used in the assessment of irritant skin reactions. Objectives: To assess the ability of eight non-invasive measurement techniques to distinguish changes in skin conditions over time, these changes being induced by five different irritants. Methods: The following techniques were compared in a multivariate analysis: laser-Doppler perfusion imaging (LDI), laser-Doppler flowmetry (LDF), transepidermal water loss (TEWL), visual scoring (VS), colorimetric measurements (Chromameter CR 200 a* and L* scales), Mexameter Hb scale (Mexa Hb) and capacitance (Corneometer CM 820). Irritants tested were sodium lauryl sulphate 2% (SLS), tape stripping (TS), tretinoin 0.05% (TRET), ultraviolet (UV) exposure to 30 W m-2 UVB/95 W m-2 UVA, and dithranol 0.5% (DIT). Measurements were performed at baseline and after 24, 48 and 72 h. The study was conducted on the upper back of 11 healthy volunteers of both sexes aged 27-51 years. Results: For DIT it was possible to discriminate over time with CR 200 a* and L*, VS, LDI, LDF and Mexa Hb. In SLS discrimination over time was seen with TEWL and LDF. Discrimination in TS was demonstrated for TEWL, VS, CR 200 a*, CM 820, LDF, LDI and Mexa Hb. In TRET discrimination ability was seen for LDI, LDF, Mexa Hb and VS. For UV it was possible to discriminate using VS, TEWL, LDF, LDI and Mexa Hb. Conclusions: Different irritation patterns need different measurement modalities in order to give optimal discrimination over time.
KW - Dithranol
KW - Multivariate analysis
KW - Sodium lauryl sulphate
KW - Tape stripping
KW - Tretinoin
KW - Ultraviolet irradiation
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U2 - 10.1046/j.1365-2133.2001.04431.x
DO - 10.1046/j.1365-2133.2001.04431.x
M3 - Article
C2 - 11736891
AN - SCOPUS:0035174989
VL - 145
SP - 696
EP - 703
JO - British Journal of Dermatology
JF - British Journal of Dermatology
SN - 0007-0963
IS - 5
ER -