TY - JOUR
T1 - Testing mitochondrial metabolic competence by cytochrome oxidase preferential cytochemistry versus immunoreactivity of subunits I and IV
AU - Fattoretti, Patrizia
AU - Balietti, Marta
AU - Giorgetti, Belinda
AU - Grossi, Yessica
AU - Casoli, Tiziana
AU - Di Stefano, Giuseppina
AU - Bertoni-Freddari, Carlo
PY - 2006/6
Y1 - 2006/6
N2 - Cytochemically evidenced COX activity was compared with levels of immunohistochemically stained mitochondrial- and nuclear-encoded subunits (CO I and CO IV) in the dentate gyrus outer molecular layer (OML) and cerebellar granular layer (GL) of adult and old rats. COX activity decreased significantly in aging, whereas CO I and CO IV levels were significantly increased both in GL and OML of old animals. These findings suggest that the age-related decay of the mitochondrial metabolic competence is not caused by a reduction of COX subunits levels, but causal events affecting mitochondria as discrete morphofunctional units of the cellular bioenergetic machinery.
AB - Cytochemically evidenced COX activity was compared with levels of immunohistochemically stained mitochondrial- and nuclear-encoded subunits (CO I and CO IV) in the dentate gyrus outer molecular layer (OML) and cerebellar granular layer (GL) of adult and old rats. COX activity decreased significantly in aging, whereas CO I and CO IV levels were significantly increased both in GL and OML of old animals. These findings suggest that the age-related decay of the mitochondrial metabolic competence is not caused by a reduction of COX subunits levels, but causal events affecting mitochondria as discrete morphofunctional units of the cellular bioenergetic machinery.
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U2 - 10.1089/rej.2006.9.215
DO - 10.1089/rej.2006.9.215
M3 - Article
C2 - 16706646
AN - SCOPUS:33744962696
VL - 9
SP - 215
EP - 218
JO - Rejuvenation Research
JF - Rejuvenation Research
SN - 1549-1684
IS - 2
ER -