Tetanus-diphtheria vaccination in adults: the long-term persistence of antibodies is not dependent on polyclonal B-cell activation and the defective response to diphtheria toxoid re-vaccination is associated to HLADRB1∗01

Claudia Ferlito, Roberto Biselli, Sabrina Mariotti, Christina von Hunolstein, Raffaela Teloni, Luisa Ralli, Antonella Pinto, Giulio Pisani, Valentina Tirelli, Michela Ileen Biondo, Gerardo Salerno, Livia Andreasi Bassi, Patrizia Lulli, Alberto Autore, Alessandro Scagliusi, Enrico Tomao, Valentina Germano, Andrea Picchianti Diamanti, Sara Caporuscio, Francesca MilanettiSimonetta Salemi, Roberto Nisini, Raffaele D'Amelio

Research output: Contribution to journalArticle

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Abstract

Cellular and humoral immune responses to tetanus-diphtheria vaccine (Td) were assessed in human leukocyte antigen (HLA)-typed Italian military personnel who received multiple concomitant vaccines. Td-specific antibodies and T-lymphocytes were measured in individuals with one (group-1) and more than one (group-2) Td boosters. A third group (group-3), who received several vaccines, but not Td, was studied to verify the hypothesis of the polyclonal B-cell activation as mechanism for antibody persistence. The antibody response to Td toxoids was higher in group-1, who showed lower baseline antibody levels, than in group-2 subjects. The antibody response to tetanus was higher than to diphtheria toxoid in both groups. No correlation between antibody and cellular response, and no interference in the response to Td by co-administration of different vaccines were observed. HLA-DRB1∗01 allele was detected at significant higher frequency in subjects unable to double the baseline anti-diphtheria antibody levels after the vaccination. Anti-tetanus and diphtheria antibodies half-lives were assessed and the long-lasting persistence above the threshold for protection (0.1 IU/ml) was estimated in over 65 and 20 years, respectively. No significant increase of anti-diphtheria antibodies was observed in consequence of polyclonal B-cell activation. This study emphasizes the duration of Td vaccination-induced seroprotection, suggesting that re-vaccination should probably be performed at intervals longer than 10 years. No reciprocal interference by concomitantly administered vaccines has been observed. HLA-DRB1∗01 allele was significantly associated with anti-diphtheria defective response. Finally, this study does not confirm that anti-diphtheria antibody levels are maintained by polyclonal B-cell activation. Clinical trial registry: The study was registered with NCT01807780.

Original languageEnglish
Pages (from-to)6718-6725
Number of pages8
JournalVaccine
Volume36
Issue number45
DOIs
Publication statusPublished - Oct 29 2018

Fingerprint

Diphtheria-Tetanus Vaccine
Diphtheria Toxoid
toxoids
Diphtheria
tetanus
Tetanus
B-lymphocytes
Vaccination
B-Lymphocytes
vaccination
vaccines
antibodies
Antibodies
Vaccines
HLA Antigens
Antibody Formation
Anti-Idiotypic Antibodies
Alleles
crossover interference
Military Personnel

Keywords

  • Anti-toxoid antibodies
  • Duration of protection
  • HLA association
  • Memory
  • Vaccination

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Tetanus-diphtheria vaccination in adults : the long-term persistence of antibodies is not dependent on polyclonal B-cell activation and the defective response to diphtheria toxoid re-vaccination is associated to HLADRB1∗01. / Ferlito, Claudia; Biselli, Roberto; Mariotti, Sabrina; von Hunolstein, Christina; Teloni, Raffaela; Ralli, Luisa; Pinto, Antonella; Pisani, Giulio; Tirelli, Valentina; Biondo, Michela Ileen; Salerno, Gerardo; Andreasi Bassi, Livia; Lulli, Patrizia; Autore, Alberto; Scagliusi, Alessandro; Tomao, Enrico; Germano, Valentina; Picchianti Diamanti, Andrea; Caporuscio, Sara; Milanetti, Francesca; Salemi, Simonetta; Nisini, Roberto; D'Amelio, Raffaele.

In: Vaccine, Vol. 36, No. 45, 29.10.2018, p. 6718-6725.

Research output: Contribution to journalArticle

Ferlito, C, Biselli, R, Mariotti, S, von Hunolstein, C, Teloni, R, Ralli, L, Pinto, A, Pisani, G, Tirelli, V, Biondo, MI, Salerno, G, Andreasi Bassi, L, Lulli, P, Autore, A, Scagliusi, A, Tomao, E, Germano, V, Picchianti Diamanti, A, Caporuscio, S, Milanetti, F, Salemi, S, Nisini, R & D'Amelio, R 2018, 'Tetanus-diphtheria vaccination in adults: the long-term persistence of antibodies is not dependent on polyclonal B-cell activation and the defective response to diphtheria toxoid re-vaccination is associated to HLADRB1∗01', Vaccine, vol. 36, no. 45, pp. 6718-6725. https://doi.org/10.1016/j.vaccine.2018.09.041
Ferlito, Claudia ; Biselli, Roberto ; Mariotti, Sabrina ; von Hunolstein, Christina ; Teloni, Raffaela ; Ralli, Luisa ; Pinto, Antonella ; Pisani, Giulio ; Tirelli, Valentina ; Biondo, Michela Ileen ; Salerno, Gerardo ; Andreasi Bassi, Livia ; Lulli, Patrizia ; Autore, Alberto ; Scagliusi, Alessandro ; Tomao, Enrico ; Germano, Valentina ; Picchianti Diamanti, Andrea ; Caporuscio, Sara ; Milanetti, Francesca ; Salemi, Simonetta ; Nisini, Roberto ; D'Amelio, Raffaele. / Tetanus-diphtheria vaccination in adults : the long-term persistence of antibodies is not dependent on polyclonal B-cell activation and the defective response to diphtheria toxoid re-vaccination is associated to HLADRB1∗01. In: Vaccine. 2018 ; Vol. 36, No. 45. pp. 6718-6725.
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abstract = "Cellular and humoral immune responses to tetanus-diphtheria vaccine (Td) were assessed in human leukocyte antigen (HLA)-typed Italian military personnel who received multiple concomitant vaccines. Td-specific antibodies and T-lymphocytes were measured in individuals with one (group-1) and more than one (group-2) Td boosters. A third group (group-3), who received several vaccines, but not Td, was studied to verify the hypothesis of the polyclonal B-cell activation as mechanism for antibody persistence. The antibody response to Td toxoids was higher in group-1, who showed lower baseline antibody levels, than in group-2 subjects. The antibody response to tetanus was higher than to diphtheria toxoid in both groups. No correlation between antibody and cellular response, and no interference in the response to Td by co-administration of different vaccines were observed. HLA-DRB1∗01 allele was detected at significant higher frequency in subjects unable to double the baseline anti-diphtheria antibody levels after the vaccination. Anti-tetanus and diphtheria antibodies half-lives were assessed and the long-lasting persistence above the threshold for protection (0.1 IU/ml) was estimated in over 65 and 20 years, respectively. No significant increase of anti-diphtheria antibodies was observed in consequence of polyclonal B-cell activation. This study emphasizes the duration of Td vaccination-induced seroprotection, suggesting that re-vaccination should probably be performed at intervals longer than 10 years. No reciprocal interference by concomitantly administered vaccines has been observed. HLA-DRB1∗01 allele was significantly associated with anti-diphtheria defective response. Finally, this study does not confirm that anti-diphtheria antibody levels are maintained by polyclonal B-cell activation. Clinical trial registry: The study was registered with NCT01807780.",
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T2 - the long-term persistence of antibodies is not dependent on polyclonal B-cell activation and the defective response to diphtheria toxoid re-vaccination is associated to HLADRB1∗01

AU - Ferlito, Claudia

AU - Biselli, Roberto

AU - Mariotti, Sabrina

AU - von Hunolstein, Christina

AU - Teloni, Raffaela

AU - Ralli, Luisa

AU - Pinto, Antonella

AU - Pisani, Giulio

AU - Tirelli, Valentina

AU - Biondo, Michela Ileen

AU - Salerno, Gerardo

AU - Andreasi Bassi, Livia

AU - Lulli, Patrizia

AU - Autore, Alberto

AU - Scagliusi, Alessandro

AU - Tomao, Enrico

AU - Germano, Valentina

AU - Picchianti Diamanti, Andrea

AU - Caporuscio, Sara

AU - Milanetti, Francesca

AU - Salemi, Simonetta

AU - Nisini, Roberto

AU - D'Amelio, Raffaele

PY - 2018/10/29

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