Tetanus toxin inhibits depolarization-induced [3H]serotonin release from rat brain cortex synaptosomes

Marco Gobbi; Francesco Facchiano; Emanuela Frittoli; Alberto Luini; Tiziana Mennini

doi:10.1016/0304-3940(93)90021-C

Tetanus toxin inhibits depolarization-induced [3H]serotonin release from rat brain cortex synaptosomes

Marco Gobbi, Francesco Facchiano, Emanuela Frittoli, Alberto Luini, Tiziana Mennini

Research output: Contribution to journal › Article › peer-review

Abstract

The effect of tetanus toxin on depolarization-induced [³H]serotonin release from superfused rat brain cortex synaptosomes was investigated. Two hours' preincubation of the synaptosomes with tetanus toxin resulted in a concentration-dependent decrease of K⁺-stimulated release, with an IC₅₀ of about 30 nM (4.5 μg/ml); this inhibitory effect was blocked by a previous incubation of the tetanus toxin with antitoxin serum. Tetanus toxin had no effect on reserpine-induced release, a model of Ca²⁺-independent release. These results indicate that tetanus toxin is able to alter the exocytotic machinery of serotoninergic terminals, in agreement with results obtained with other neurotransmitters. They also indicate that serotoninergic terminals possess the receptor for tetanus toxin. These findings are in line with in vivo observations suggesting a role for serotoninergic system in tetanus intoxication.

Original language	English
Pages (from-to)	205-208
Number of pages	4
Journal	Neuroscience Letters
Volume	151
Issue number	2
DOIs	https://doi.org/10.1016/0304-3940(93)90021-C
Publication status	Published - Mar 19 1993

Keywords

Rat brain
Release
Serotonin
Synaptosome
Tetanus toxin

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/0304-3940(93)90021-C

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title = "Tetanus toxin inhibits depolarization-induced [3H]serotonin release from rat brain cortex synaptosomes",

abstract = "The effect of tetanus toxin on depolarization-induced [3H]serotonin release from superfused rat brain cortex synaptosomes was investigated. Two hours' preincubation of the synaptosomes with tetanus toxin resulted in a concentration-dependent decrease of K+-stimulated release, with an IC50 of about 30 nM (4.5 μg/ml); this inhibitory effect was blocked by a previous incubation of the tetanus toxin with antitoxin serum. Tetanus toxin had no effect on reserpine-induced release, a model of Ca2+-independent release. These results indicate that tetanus toxin is able to alter the exocytotic machinery of serotoninergic terminals, in agreement with results obtained with other neurotransmitters. They also indicate that serotoninergic terminals possess the receptor for tetanus toxin. These findings are in line with in vivo observations suggesting a role for serotoninergic system in tetanus intoxication.",

keywords = "Rat brain, Release, Serotonin, Synaptosome, Tetanus toxin",

author = "Marco Gobbi and Francesco Facchiano and Emanuela Frittoli and Alberto Luini and Tiziana Mennini",

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T1 - Tetanus toxin inhibits depolarization-induced [3H]serotonin release from rat brain cortex synaptosomes

AU - Gobbi, Marco

AU - Facchiano, Francesco

AU - Frittoli, Emanuela

AU - Luini, Alberto

AU - Mennini, Tiziana

PY - 1993/3/19

Y1 - 1993/3/19

N2 - The effect of tetanus toxin on depolarization-induced [3H]serotonin release from superfused rat brain cortex synaptosomes was investigated. Two hours' preincubation of the synaptosomes with tetanus toxin resulted in a concentration-dependent decrease of K+-stimulated release, with an IC50 of about 30 nM (4.5 μg/ml); this inhibitory effect was blocked by a previous incubation of the tetanus toxin with antitoxin serum. Tetanus toxin had no effect on reserpine-induced release, a model of Ca2+-independent release. These results indicate that tetanus toxin is able to alter the exocytotic machinery of serotoninergic terminals, in agreement with results obtained with other neurotransmitters. They also indicate that serotoninergic terminals possess the receptor for tetanus toxin. These findings are in line with in vivo observations suggesting a role for serotoninergic system in tetanus intoxication.

AB - The effect of tetanus toxin on depolarization-induced [3H]serotonin release from superfused rat brain cortex synaptosomes was investigated. Two hours' preincubation of the synaptosomes with tetanus toxin resulted in a concentration-dependent decrease of K+-stimulated release, with an IC50 of about 30 nM (4.5 μg/ml); this inhibitory effect was blocked by a previous incubation of the tetanus toxin with antitoxin serum. Tetanus toxin had no effect on reserpine-induced release, a model of Ca2+-independent release. These results indicate that tetanus toxin is able to alter the exocytotic machinery of serotoninergic terminals, in agreement with results obtained with other neurotransmitters. They also indicate that serotoninergic terminals possess the receptor for tetanus toxin. These findings are in line with in vivo observations suggesting a role for serotoninergic system in tetanus intoxication.

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KW - Release

KW - Serotonin

KW - Synaptosome

KW - Tetanus toxin

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