TGFβ-induced EMT requires focal adhesion kinase (FAK) signaling

Carla Cicchini, Ilaria Laudadio, Franca Citarella, Marco Corazzari, Corinna Steindler, Alice Conigliaro, Antonio Fantoni, Laura Amicone, Marco Tripodi

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

The epithelial-to-mesenchymal transition (EMT) is a crucial process, occurring both during development and tumor progression, by which an epithelial cell undergoes a conversion to a mesenchymal phenotype, dissociates from initial contacts and migrates to secondary sites. We recently reported that in hepatocytes the multifunctional cytokine TGFβ induces a full EMT characterized by (i) Snail induction, (ii) E-cadherin delocalization and down-regulation, (iii) down-regulation of the hepatocyte transcriptional factor HNF4α and (iv) up-regulation of mesenchymal and invasiveness markers. In particular, we showed that Snail directly causes the transcriptional down-regulation of E-cadherin and HNF4, while it is not sufficient for the up-regulation of mesenchymal and invasiveness EMT markers. In this paper, we show that in hepatocytes TGFβ induces a Src-dependent activation of the focal adhesion protein FAK. More relevantly, we gathered results indicating that FAK signaling is required for (i) transcriptional up-regulation of mesenchymal and invasiveness markers and (ii) delocalization of membrane-bound E-cadherin. Our results provide the first evidence of FAK functional role in TGFβ-mediated EMT in hepatocytes.

Original languageEnglish
Pages (from-to)143-152
Number of pages10
JournalExperimental Cell Research
Volume314
Issue number1
DOIs
Publication statusPublished - Jan 1 2008

Fingerprint

Focal Adhesion Protein-Tyrosine Kinases
Epithelial-Mesenchymal Transition
Hepatocytes
Cadherins
Up-Regulation
Down-Regulation
Focal Adhesions
Snails
Protein Kinases
Epithelial Cells
Cytokines
Phenotype
Membranes
Neoplasms

Keywords

  • FAK
  • MT
  • Src
  • TGFβ

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cicchini, C., Laudadio, I., Citarella, F., Corazzari, M., Steindler, C., Conigliaro, A., ... Tripodi, M. (2008). TGFβ-induced EMT requires focal adhesion kinase (FAK) signaling. Experimental Cell Research, 314(1), 143-152. https://doi.org/10.1016/j.yexcr.2007.09.005

TGFβ-induced EMT requires focal adhesion kinase (FAK) signaling. / Cicchini, Carla; Laudadio, Ilaria; Citarella, Franca; Corazzari, Marco; Steindler, Corinna; Conigliaro, Alice; Fantoni, Antonio; Amicone, Laura; Tripodi, Marco.

In: Experimental Cell Research, Vol. 314, No. 1, 01.01.2008, p. 143-152.

Research output: Contribution to journalArticle

Cicchini, C, Laudadio, I, Citarella, F, Corazzari, M, Steindler, C, Conigliaro, A, Fantoni, A, Amicone, L & Tripodi, M 2008, 'TGFβ-induced EMT requires focal adhesion kinase (FAK) signaling', Experimental Cell Research, vol. 314, no. 1, pp. 143-152. https://doi.org/10.1016/j.yexcr.2007.09.005
Cicchini C, Laudadio I, Citarella F, Corazzari M, Steindler C, Conigliaro A et al. TGFβ-induced EMT requires focal adhesion kinase (FAK) signaling. Experimental Cell Research. 2008 Jan 1;314(1):143-152. https://doi.org/10.1016/j.yexcr.2007.09.005
Cicchini, Carla ; Laudadio, Ilaria ; Citarella, Franca ; Corazzari, Marco ; Steindler, Corinna ; Conigliaro, Alice ; Fantoni, Antonio ; Amicone, Laura ; Tripodi, Marco. / TGFβ-induced EMT requires focal adhesion kinase (FAK) signaling. In: Experimental Cell Research. 2008 ; Vol. 314, No. 1. pp. 143-152.
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