TGF-β1 differentially modulates the collagen VI α5 and α6 chains in human tendon cultures.

Patrizia Sabatelli, Francesca Rita Sardone, Francesco Traina, L. Merlini, Spartaco Santi, R. Wagener, Cesare Faldini

Research output: Contribution to journalArticle

Abstract

Collagen VI is a microfibrillar collagen with a potential regulatory role in tendon repair mechanism. We studied the expression of collagen VI α5 and α6 chains in normal human tendon fibroblast cultures, both under basal condition and in response to TGF-β1, a potent regulator of tendon healing. Under basal condition, we found that the α5 chain was expressed, although to a lesser extent with respect to the α3 chain; in contrast, the α6 chain was absent. The treatment with TGFβ1 induced an opposite effect on the expression of the α5 and α6 chains; in fact, while the α5 chain was dramatically reduced, the α6 chain was induced and released in the culture medium. These data indicate that collagen VI α5 and α6 chains are differentially involved in tendon matrix homeostasis. The α6 chain may represent a new potential biomarker for monitoring TGFβ1-related events in tendon, as healing and fibrotic scar formation.
Original languageEnglish
Pages (from-to)107-113
Number of pages7
JournalJournal of Biological Regulators and Homeostatic Agents
Publication statusPublished - 2016

Fingerprint

Tendons
Collagen
Cicatrix
Culture Media
Homeostasis
Fibroblasts
Biomarkers

Cite this

TGF-β1 differentially modulates the collagen VI α5 and α6 chains in human tendon cultures. / Sabatelli, Patrizia; Sardone, Francesca Rita; Traina, Francesco; Merlini, L.; Santi, Spartaco; Wagener, R.; Faldini, Cesare.

In: Journal of Biological Regulators and Homeostatic Agents, 2016, p. 107-113.

Research output: Contribution to journalArticle

@article{a184b2c4284b45ec9bc95af92cd41964,
title = "TGF-β1 differentially modulates the collagen VI α5 and α6 chains in human tendon cultures.",
abstract = "Collagen VI is a microfibrillar collagen with a potential regulatory role in tendon repair mechanism. We studied the expression of collagen VI α5 and α6 chains in normal human tendon fibroblast cultures, both under basal condition and in response to TGF-β1, a potent regulator of tendon healing. Under basal condition, we found that the α5 chain was expressed, although to a lesser extent with respect to the α3 chain; in contrast, the α6 chain was absent. The treatment with TGFβ1 induced an opposite effect on the expression of the α5 and α6 chains; in fact, while the α5 chain was dramatically reduced, the α6 chain was induced and released in the culture medium. These data indicate that collagen VI α5 and α6 chains are differentially involved in tendon matrix homeostasis. The α6 chain may represent a new potential biomarker for monitoring TGFβ1-related events in tendon, as healing and fibrotic scar formation.",
author = "Patrizia Sabatelli and Sardone, {Francesca Rita} and Francesco Traina and L. Merlini and Spartaco Santi and R. Wagener and Cesare Faldini",
year = "2016",
language = "English",
pages = "107--113",
journal = "Journal of Biological Regulators and Homeostatic Agents",
issn = "0393-974X",
publisher = "Biolife s.a.s.",

}

TY - JOUR

T1 - TGF-β1 differentially modulates the collagen VI α5 and α6 chains in human tendon cultures.

AU - Sabatelli, Patrizia

AU - Sardone, Francesca Rita

AU - Traina, Francesco

AU - Merlini, L.

AU - Santi, Spartaco

AU - Wagener, R.

AU - Faldini, Cesare

PY - 2016

Y1 - 2016

N2 - Collagen VI is a microfibrillar collagen with a potential regulatory role in tendon repair mechanism. We studied the expression of collagen VI α5 and α6 chains in normal human tendon fibroblast cultures, both under basal condition and in response to TGF-β1, a potent regulator of tendon healing. Under basal condition, we found that the α5 chain was expressed, although to a lesser extent with respect to the α3 chain; in contrast, the α6 chain was absent. The treatment with TGFβ1 induced an opposite effect on the expression of the α5 and α6 chains; in fact, while the α5 chain was dramatically reduced, the α6 chain was induced and released in the culture medium. These data indicate that collagen VI α5 and α6 chains are differentially involved in tendon matrix homeostasis. The α6 chain may represent a new potential biomarker for monitoring TGFβ1-related events in tendon, as healing and fibrotic scar formation.

AB - Collagen VI is a microfibrillar collagen with a potential regulatory role in tendon repair mechanism. We studied the expression of collagen VI α5 and α6 chains in normal human tendon fibroblast cultures, both under basal condition and in response to TGF-β1, a potent regulator of tendon healing. Under basal condition, we found that the α5 chain was expressed, although to a lesser extent with respect to the α3 chain; in contrast, the α6 chain was absent. The treatment with TGFβ1 induced an opposite effect on the expression of the α5 and α6 chains; in fact, while the α5 chain was dramatically reduced, the α6 chain was induced and released in the culture medium. These data indicate that collagen VI α5 and α6 chains are differentially involved in tendon matrix homeostasis. The α6 chain may represent a new potential biomarker for monitoring TGFβ1-related events in tendon, as healing and fibrotic scar formation.

M3 - Article

SP - 107

EP - 113

JO - Journal of Biological Regulators and Homeostatic Agents

JF - Journal of Biological Regulators and Homeostatic Agents

SN - 0393-974X

ER -