TGF-β1 Overexpression in Murine Pancreas Induces Chronic Pancreatitis and Together with TNF-α, Triggers Insulin-Dependent Diabetes

F. Sanvito, A. Nichols, P. L. Herrera, J. Huarte, A. Wohlwend, J. D. Vassalli, L. Orci

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

We have generated transgenic mice overexpressing TGF-β1 in pancreatic β cells. This resulted in massive fibrosis of the pancreas; in adult mice, most of the acini were replaced by fibrotic and adipose tissues. A conspicuous disorganisation of the islets of Langerhans was also observed, however, the number of β cells was not decreased and the mice were normoglycemic. Backcrossing to transgenic mice overexpressing TNF-α in their islet β cells (which also remain normoglycemic, (1)) yielded double transgenics, most of which became diabetic by the age of 4 months; histological analysis revealed a dramatic decrease in insulin-containing β cells. (Part of this work has been reported in abstract form, Experientia 51, p. A9, February 1995).

Original languageEnglish
Pages (from-to)1279-1286
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume217
Issue number3
DOIs
Publication statusPublished - Dec 26 1995

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Chronic Pancreatitis
Medical problems
Islets of Langerhans
Transgenic Mice
Pancreas
Insulin
Tissue
Inbreeding
Adipose Tissue
Fibrosis
Cell Count

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Biophysics
  • Biochemistry

Cite this

TGF-β1 Overexpression in Murine Pancreas Induces Chronic Pancreatitis and Together with TNF-α, Triggers Insulin-Dependent Diabetes. / Sanvito, F.; Nichols, A.; Herrera, P. L.; Huarte, J.; Wohlwend, A.; Vassalli, J. D.; Orci, L.

In: Biochemical and Biophysical Research Communications, Vol. 217, No. 3, 26.12.1995, p. 1279-1286.

Research output: Contribution to journalArticle

Sanvito, F. ; Nichols, A. ; Herrera, P. L. ; Huarte, J. ; Wohlwend, A. ; Vassalli, J. D. ; Orci, L. / TGF-β1 Overexpression in Murine Pancreas Induces Chronic Pancreatitis and Together with TNF-α, Triggers Insulin-Dependent Diabetes. In: Biochemical and Biophysical Research Communications. 1995 ; Vol. 217, No. 3. pp. 1279-1286.
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