Thapsigarin induces 78K GRP in human TE671/RD cells

A. Delpino, V. Lapenta, P. Piselli, S. Fucile

Research output: Contribution to journalArticle

Abstract

The effects of thapsigargin (TG) on the homeostasis of cytosolic Ca2+ and on the protein synthesis were investigated in TE671/RD (human rabdomyosarcoma) cells. TE671/RD cells exhibited a long lasting rise of cytosolic free Ca2+ concentration [Ca2+](i) after TG (100 nM) bath application. TG was more efficient as compared to acetylcholine (ACh, 10 μM) in rising [Ca2+](i). The overall protein synthetic activity was inhibited by TG: 3H-leucine incorporation was reduced to about 60% of control level in cells treated for 20 min with 100 nM TG, while following 1 hr exposure to 1 μM TG the amount of leucine incorporated into proteins was only 28% of control. Anyway, even after a very strong treatment (1 μM TG for 1 hr) the protein synthetic activity partially resumed when cells were allowed to recover in drug-free medium. Furthermore, TG treatment selectively induced the expression of a 78 kd protein, identified with the 78 kd glucose - regulated protein (78K GRP). The level of induction was dose and time-dependent and maximal 78K GRP expression was obtained at TG concentration of 100 nM for 10 min.

Original languageEnglish
Pages (from-to)373-379
Number of pages7
JournalJournal of Experimental and Clinical Cancer Research
Volume13
Issue number4
Publication statusPublished - 1994

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Keywords

  • 78K GRP
  • Cytosolic Ca
  • Thapsigargin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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