The γ subunit of the interleukin-2 receptor is expressed in human monocytes and modulated by interleukin-2, interferon γ, and transforming growth factor β1

Maria Carla Bosco, Igor Espinoza-Delgado, Michael Schwabe, Sarah M. Russell, Warren J. Leonard, Dan L. Longo, Luigi Varesio

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The interleukin-2 receptor γ (IL-2Rγ) chain is a newly recognized component of the IL-2R of lymphoid cells that is required for their response to IL-2. We investigated the expression of IL-2Rγ protein in human monocytes by Western blot analysis using an antiserum specific for IL-2Rγ. We found that IL-2Rγ subunit is constitutively expressed in human monocytes and upregulated by the monocyte-activating factors IL-2 and interferon γ (IFNγ). Furthermore, we show that transforming growth factor β1 (TGFβ1) downmodulates, in a dose-dependent manner, basal and IL-2-induced, but not IFNγ-induced, IL-2Rγ chain expression, and this effect may be responsible for TGFβ1 suppressive activity on IL-2-activated monocytes. Overall, these results show that the expression of the IL-2Rγ subunit in human monocytes is tightly regulated by the cytokine network, suggesting a critical role played by this protein on monocyte activation.

Original languageEnglish
Pages (from-to)3462-3467
Number of pages6
JournalBlood
Volume83
Issue number12
Publication statusPublished - Jun 15 1994

Fingerprint

Interleukin-2 Receptors
Transforming Growth Factors
Interferons
Interleukin-2
Monocytes
Immune Sera
Proteins
Western Blotting
Chemical activation
Lymphocytes
Cytokines

ASJC Scopus subject areas

  • Hematology

Cite this

The γ subunit of the interleukin-2 receptor is expressed in human monocytes and modulated by interleukin-2, interferon γ, and transforming growth factor β1 . / Bosco, Maria Carla; Espinoza-Delgado, Igor; Schwabe, Michael; Russell, Sarah M.; Leonard, Warren J.; Longo, Dan L.; Varesio, Luigi.

In: Blood, Vol. 83, No. 12, 15.06.1994, p. 3462-3467.

Research output: Contribution to journalArticle

Bosco, Maria Carla ; Espinoza-Delgado, Igor ; Schwabe, Michael ; Russell, Sarah M. ; Leonard, Warren J. ; Longo, Dan L. ; Varesio, Luigi. / The γ subunit of the interleukin-2 receptor is expressed in human monocytes and modulated by interleukin-2, interferon γ, and transforming growth factor β1 . In: Blood. 1994 ; Vol. 83, No. 12. pp. 3462-3467.
@article{47d50918ef3f48caab4e6901364d6a1e,
title = "The γ subunit of the interleukin-2 receptor is expressed in human monocytes and modulated by interleukin-2, interferon γ, and transforming growth factor β1",
abstract = "The interleukin-2 receptor γ (IL-2Rγ) chain is a newly recognized component of the IL-2R of lymphoid cells that is required for their response to IL-2. We investigated the expression of IL-2Rγ protein in human monocytes by Western blot analysis using an antiserum specific for IL-2Rγ. We found that IL-2Rγ subunit is constitutively expressed in human monocytes and upregulated by the monocyte-activating factors IL-2 and interferon γ (IFNγ). Furthermore, we show that transforming growth factor β1 (TGFβ1) downmodulates, in a dose-dependent manner, basal and IL-2-induced, but not IFNγ-induced, IL-2Rγ chain expression, and this effect may be responsible for TGFβ1 suppressive activity on IL-2-activated monocytes. Overall, these results show that the expression of the IL-2Rγ subunit in human monocytes is tightly regulated by the cytokine network, suggesting a critical role played by this protein on monocyte activation.",
author = "Bosco, {Maria Carla} and Igor Espinoza-Delgado and Michael Schwabe and Russell, {Sarah M.} and Leonard, {Warren J.} and Longo, {Dan L.} and Luigi Varesio",
year = "1994",
month = "6",
day = "15",
language = "English",
volume = "83",
pages = "3462--3467",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "12",

}

TY - JOUR

T1 - The γ subunit of the interleukin-2 receptor is expressed in human monocytes and modulated by interleukin-2, interferon γ, and transforming growth factor β1

AU - Bosco, Maria Carla

AU - Espinoza-Delgado, Igor

AU - Schwabe, Michael

AU - Russell, Sarah M.

AU - Leonard, Warren J.

AU - Longo, Dan L.

AU - Varesio, Luigi

PY - 1994/6/15

Y1 - 1994/6/15

N2 - The interleukin-2 receptor γ (IL-2Rγ) chain is a newly recognized component of the IL-2R of lymphoid cells that is required for their response to IL-2. We investigated the expression of IL-2Rγ protein in human monocytes by Western blot analysis using an antiserum specific for IL-2Rγ. We found that IL-2Rγ subunit is constitutively expressed in human monocytes and upregulated by the monocyte-activating factors IL-2 and interferon γ (IFNγ). Furthermore, we show that transforming growth factor β1 (TGFβ1) downmodulates, in a dose-dependent manner, basal and IL-2-induced, but not IFNγ-induced, IL-2Rγ chain expression, and this effect may be responsible for TGFβ1 suppressive activity on IL-2-activated monocytes. Overall, these results show that the expression of the IL-2Rγ subunit in human monocytes is tightly regulated by the cytokine network, suggesting a critical role played by this protein on monocyte activation.

AB - The interleukin-2 receptor γ (IL-2Rγ) chain is a newly recognized component of the IL-2R of lymphoid cells that is required for their response to IL-2. We investigated the expression of IL-2Rγ protein in human monocytes by Western blot analysis using an antiserum specific for IL-2Rγ. We found that IL-2Rγ subunit is constitutively expressed in human monocytes and upregulated by the monocyte-activating factors IL-2 and interferon γ (IFNγ). Furthermore, we show that transforming growth factor β1 (TGFβ1) downmodulates, in a dose-dependent manner, basal and IL-2-induced, but not IFNγ-induced, IL-2Rγ chain expression, and this effect may be responsible for TGFβ1 suppressive activity on IL-2-activated monocytes. Overall, these results show that the expression of the IL-2Rγ subunit in human monocytes is tightly regulated by the cytokine network, suggesting a critical role played by this protein on monocyte activation.

UR - http://www.scopus.com/inward/record.url?scp=0028229419&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028229419&partnerID=8YFLogxK

M3 - Article

VL - 83

SP - 3462

EP - 3467

JO - Blood

JF - Blood

SN - 0006-4971

IS - 12

ER -