Abstract
We report the results of an external quality control program, including 17 Italian centers involved in the care of patients infected by HIV, to evaluate CD4 T cell count proficiency and reproducibility. The centers received two commercial stabilized blood preparations, one with "normal" and one with "low" CD4 T cell content. The centers were asked to process the samples two times, 1 week apart, with the same procedure used for samples from HIV patients. Most centers showed a good performance of CD4 frequency and absolute count determinations. In particular, the "low" sample was correctly analyzed by all centers; only two underestimated the "normal" sample CD4 frequency, and only one underestimated the CD4 absolute count by >100 CD4 cells/μl. Overall, our data suggest that most Italian laboratories provide reliable and reproducible results in evaluating CD4 T cells in HIV+ samples.
| Original language | English |
|---|---|
| Pages (from-to) | 777-781 |
| Number of pages | 5 |
| Journal | AIDS Research and Human Retroviruses |
| Volume | 23 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 2007 |
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ASJC Scopus subject areas
- Immunology
- Virology
Cite this
The 2005 Italian quality control study for the evaluation of CD4 cells in centers involved in the treatment of HIV type 1 patients. / Martini, Federico; D'Offizi, Gianpiero; Girardi, Enrico; Vitale, Alessia; Cimini, Eleonora; Mollicone, Bianca; Vlassi, Crysoula; Aiuti, Fernando; Atripaldi, L.; Bodini, U.; De Francesco, D.; De Soccio, G.; Gallo, L.; Gentileschi, E.; Giudizi, M. G.; Martini, C.; Mordacchini, M.; Moroni, G. A.; Pagliaro, P. P.; Piras, S.; Soldini, L.; Vanacore, R.; Vullo, V.
In: AIDS Research and Human Retroviruses, Vol. 23, No. 6, 06.2007, p. 777-781.Research output: Contribution to journal › Article
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TY - JOUR
T1 - The 2005 Italian quality control study for the evaluation of CD4 cells in centers involved in the treatment of HIV type 1 patients
AU - Martini, Federico
AU - D'Offizi, Gianpiero
AU - Girardi, Enrico
AU - Vitale, Alessia
AU - Cimini, Eleonora
AU - Mollicone, Bianca
AU - Vlassi, Crysoula
AU - Aiuti, Fernando
AU - Atripaldi, L.
AU - Bodini, U.
AU - De Francesco, D.
AU - De Soccio, G.
AU - Gallo, L.
AU - Gentileschi, E.
AU - Giudizi, M. G.
AU - Martini, C.
AU - Mordacchini, M.
AU - Moroni, G. A.
AU - Pagliaro, P. P.
AU - Piras, S.
AU - Soldini, L.
AU - Vanacore, R.
AU - Vullo, V.
PY - 2007/6
Y1 - 2007/6
N2 - We report the results of an external quality control program, including 17 Italian centers involved in the care of patients infected by HIV, to evaluate CD4 T cell count proficiency and reproducibility. The centers received two commercial stabilized blood preparations, one with "normal" and one with "low" CD4 T cell content. The centers were asked to process the samples two times, 1 week apart, with the same procedure used for samples from HIV patients. Most centers showed a good performance of CD4 frequency and absolute count determinations. In particular, the "low" sample was correctly analyzed by all centers; only two underestimated the "normal" sample CD4 frequency, and only one underestimated the CD4 absolute count by >100 CD4 cells/μl. Overall, our data suggest that most Italian laboratories provide reliable and reproducible results in evaluating CD4 T cells in HIV+ samples.
AB - We report the results of an external quality control program, including 17 Italian centers involved in the care of patients infected by HIV, to evaluate CD4 T cell count proficiency and reproducibility. The centers received two commercial stabilized blood preparations, one with "normal" and one with "low" CD4 T cell content. The centers were asked to process the samples two times, 1 week apart, with the same procedure used for samples from HIV patients. Most centers showed a good performance of CD4 frequency and absolute count determinations. In particular, the "low" sample was correctly analyzed by all centers; only two underestimated the "normal" sample CD4 frequency, and only one underestimated the CD4 absolute count by >100 CD4 cells/μl. Overall, our data suggest that most Italian laboratories provide reliable and reproducible results in evaluating CD4 T cells in HIV+ samples.
UR - http://www.scopus.com/inward/record.url?scp=34447289386&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34447289386&partnerID=8YFLogxK
U2 - 10.1089/aid.2006.0295
DO - 10.1089/aid.2006.0295
M3 - Article
C2 - 17604540
AN - SCOPUS:34447289386
VL - 23
SP - 777
EP - 781
JO - AIDS Research and Human Retroviruses
JF - AIDS Research and Human Retroviruses
SN - 0889-2229
IS - 6
ER -