Abstract
The primary and secondary structures of the 23 S ribosomal RNA of the archaeon (archeabacterium) Sulfolobus solfaxaricus strain MT4, were deduced from the sequence of the cloned gene. The structure of the 'processing stem' of the 23 S rRNA was also deduced and the position of some processing cuts was experimentally determined. Sulfolobus solfataricus 23 S rRNA was found to resemble closely in both sequence and structure the 23 S rRNAs of other thermophilic, sulphur-dependent archaea (crenarchaeota), particularly that of Desulfurococcus mobilis. However, ribosome reconstitution experiments demonstrated that the 23 S rRNAs of Sulfolobus and Desulfurococcus were not functionally interchangeable, possibly because of subtle but crucial differences in a region involved in the binding of an assembly-initiating protein.
Original language | English |
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Pages (from-to) | 170-176 |
Number of pages | 7 |
Journal | Systematic and Applied Microbiology |
Volume | 16 |
Issue number | 2 |
Publication status | Published - 1993 |
Keywords
- 23 S rRNA secondary structure
- Archaea
- Crenarchaeota
- Extreme thermophiles
- Ribosome reconstitution
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology