The 5-lipoxygenase enzymatic pathway in the mouse brain: Young versus old

Cinzia M. Chinnici, Yuemang Yao, Domenico Praticò

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

The 5-lipoxygenase (5-LO) pathway of arachidonic acid metabolism is an important source of inflammatory mediators, and is also present in the central nervous system. In this study, we assayed the expression levels and activity of 5-LO in different brain regions of young versus old C57Bl/6 mice. 5-LO mRNA was expressed in all the brain regions considered, i.e. cortex, hippocampus, and cerebellum. The highest expression level was observed in cerebellum compared with cortex and hippocampus, although it was not affected by aging. By contrast, 5-LO mRNA and protein levels were significantly increased in the hippocampus of old (25 months) versus young (3 months) mice. Finally, levels of the leukotriene B4, a metabolic product of 5-LO, were significantly increased in the hippocampus of old mice, but no difference was observed in cortex and cerebellum. These results demonstrate that the 5-LO enzymatic pathway is widely expressed in the mouse CNS, where significant changes are region-specific and age-dependent. This observation supports the hypothesis that 5-LO may be involved in diseases of the brain associated with aging.

Original languageEnglish
Pages (from-to)1457-1462
Number of pages6
JournalNeurobiology of Aging
Volume28
Issue number9
DOIs
Publication statusPublished - Sep 2007

Fingerprint

Arachidonate 5-Lipoxygenase
Brain
Hippocampus
Cerebellum
Messenger RNA
Leukotriene B4
Brain Diseases
Central Nervous System

Keywords

  • Aging
  • Inflammation
  • Leukotrienes
  • Lipoxygenase

ASJC Scopus subject areas

  • Clinical Neurology
  • Biological Psychiatry
  • Developmental Neuroscience
  • Neurology
  • Psychology(all)

Cite this

The 5-lipoxygenase enzymatic pathway in the mouse brain : Young versus old. / Chinnici, Cinzia M.; Yao, Yuemang; Praticò, Domenico.

In: Neurobiology of Aging, Vol. 28, No. 9, 09.2007, p. 1457-1462.

Research output: Contribution to journalArticle

Chinnici, Cinzia M. ; Yao, Yuemang ; Praticò, Domenico. / The 5-lipoxygenase enzymatic pathway in the mouse brain : Young versus old. In: Neurobiology of Aging. 2007 ; Vol. 28, No. 9. pp. 1457-1462.
@article{421c760845c64b7eac881cee08d99bea,
title = "The 5-lipoxygenase enzymatic pathway in the mouse brain: Young versus old",
abstract = "The 5-lipoxygenase (5-LO) pathway of arachidonic acid metabolism is an important source of inflammatory mediators, and is also present in the central nervous system. In this study, we assayed the expression levels and activity of 5-LO in different brain regions of young versus old C57Bl/6 mice. 5-LO mRNA was expressed in all the brain regions considered, i.e. cortex, hippocampus, and cerebellum. The highest expression level was observed in cerebellum compared with cortex and hippocampus, although it was not affected by aging. By contrast, 5-LO mRNA and protein levels were significantly increased in the hippocampus of old (25 months) versus young (3 months) mice. Finally, levels of the leukotriene B4, a metabolic product of 5-LO, were significantly increased in the hippocampus of old mice, but no difference was observed in cortex and cerebellum. These results demonstrate that the 5-LO enzymatic pathway is widely expressed in the mouse CNS, where significant changes are region-specific and age-dependent. This observation supports the hypothesis that 5-LO may be involved in diseases of the brain associated with aging.",
keywords = "Aging, Inflammation, Leukotrienes, Lipoxygenase",
author = "Chinnici, {Cinzia M.} and Yuemang Yao and Domenico Pratic{\`o}",
year = "2007",
month = "9",
doi = "10.1016/j.neurobiolaging.2006.06.007",
language = "English",
volume = "28",
pages = "1457--1462",
journal = "Neurobiology of Aging",
issn = "0197-4580",
publisher = "Elsevier Inc.",
number = "9",

}

TY - JOUR

T1 - The 5-lipoxygenase enzymatic pathway in the mouse brain

T2 - Young versus old

AU - Chinnici, Cinzia M.

AU - Yao, Yuemang

AU - Praticò, Domenico

PY - 2007/9

Y1 - 2007/9

N2 - The 5-lipoxygenase (5-LO) pathway of arachidonic acid metabolism is an important source of inflammatory mediators, and is also present in the central nervous system. In this study, we assayed the expression levels and activity of 5-LO in different brain regions of young versus old C57Bl/6 mice. 5-LO mRNA was expressed in all the brain regions considered, i.e. cortex, hippocampus, and cerebellum. The highest expression level was observed in cerebellum compared with cortex and hippocampus, although it was not affected by aging. By contrast, 5-LO mRNA and protein levels were significantly increased in the hippocampus of old (25 months) versus young (3 months) mice. Finally, levels of the leukotriene B4, a metabolic product of 5-LO, were significantly increased in the hippocampus of old mice, but no difference was observed in cortex and cerebellum. These results demonstrate that the 5-LO enzymatic pathway is widely expressed in the mouse CNS, where significant changes are region-specific and age-dependent. This observation supports the hypothesis that 5-LO may be involved in diseases of the brain associated with aging.

AB - The 5-lipoxygenase (5-LO) pathway of arachidonic acid metabolism is an important source of inflammatory mediators, and is also present in the central nervous system. In this study, we assayed the expression levels and activity of 5-LO in different brain regions of young versus old C57Bl/6 mice. 5-LO mRNA was expressed in all the brain regions considered, i.e. cortex, hippocampus, and cerebellum. The highest expression level was observed in cerebellum compared with cortex and hippocampus, although it was not affected by aging. By contrast, 5-LO mRNA and protein levels were significantly increased in the hippocampus of old (25 months) versus young (3 months) mice. Finally, levels of the leukotriene B4, a metabolic product of 5-LO, were significantly increased in the hippocampus of old mice, but no difference was observed in cortex and cerebellum. These results demonstrate that the 5-LO enzymatic pathway is widely expressed in the mouse CNS, where significant changes are region-specific and age-dependent. This observation supports the hypothesis that 5-LO may be involved in diseases of the brain associated with aging.

KW - Aging

KW - Inflammation

KW - Leukotrienes

KW - Lipoxygenase

UR - http://www.scopus.com/inward/record.url?scp=34447565153&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34447565153&partnerID=8YFLogxK

U2 - 10.1016/j.neurobiolaging.2006.06.007

DO - 10.1016/j.neurobiolaging.2006.06.007

M3 - Article

C2 - 16930777

AN - SCOPUS:34447565153

VL - 28

SP - 1457

EP - 1462

JO - Neurobiology of Aging

JF - Neurobiology of Aging

SN - 0197-4580

IS - 9

ER -