The 5-lipoxygenase enzymatic pathway in the mouse brain: Young versus old

Cinzia M. Chinnici, Yuemang Yao, Domenico Praticò

Research output: Contribution to journalArticle

Abstract

The 5-lipoxygenase (5-LO) pathway of arachidonic acid metabolism is an important source of inflammatory mediators, and is also present in the central nervous system. In this study, we assayed the expression levels and activity of 5-LO in different brain regions of young versus old C57Bl/6 mice. 5-LO mRNA was expressed in all the brain regions considered, i.e. cortex, hippocampus, and cerebellum. The highest expression level was observed in cerebellum compared with cortex and hippocampus, although it was not affected by aging. By contrast, 5-LO mRNA and protein levels were significantly increased in the hippocampus of old (25 months) versus young (3 months) mice. Finally, levels of the leukotriene B4, a metabolic product of 5-LO, were significantly increased in the hippocampus of old mice, but no difference was observed in cortex and cerebellum. These results demonstrate that the 5-LO enzymatic pathway is widely expressed in the mouse CNS, where significant changes are region-specific and age-dependent. This observation supports the hypothesis that 5-LO may be involved in diseases of the brain associated with aging.

Original languageEnglish
Pages (from-to)1457-1462
Number of pages6
JournalNeurobiology of Aging
Volume28
Issue number9
DOIs
Publication statusPublished - Sep 2007

Keywords

  • Aging
  • Inflammation
  • Leukotrienes
  • Lipoxygenase

ASJC Scopus subject areas

  • Clinical Neurology
  • Biological Psychiatry
  • Developmental Neuroscience
  • Neurology
  • Psychology(all)

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