The abnormal oocyte phenotype is correlated with the presence of blood transposon in Drosophila melanogaster.

G. Lavorgna, C. Malva, A. Manzi, S. Gigliotti, F. Graziani

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The abnormal oocyte mutation (2;44) originates in the wild: it confers no visible phenotype on homozygous abo males or females, but homozygous abo females produce defective eggs and the probability of their developing into adults is much lower than that of heterozygous sister females. We isolated by chromosome walking 200 kb of DNA from region 32. This paper reports that a restriction enzyme site polymorphism analysis in wild type and mutant stocks allowed us to identify a DNA rearrangement present only in stocks carrying the abo mutation. The rearrangement is caused by a DNA insert on the abo chromosome in region 32E which, by restriction map and sequence analysis, was identified as copia-like blood transposon. The transposon, in strains that had remained in abo homozygous conditions for several generations and had lost the abo maternal-effect, was no longer present in region 32E. Certain features of the abo mutation, discussed in the light of this finding, may be ascribed to the nature of the particular allele studied.

Original languageEnglish
Pages (from-to)485-494
Number of pages10
JournalGenetics
Volume123
Issue number3
Publication statusPublished - Nov 1989

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Drosophila melanogaster
Oocytes
Phenotype
Mutation
Chromosome Walking
Gene Rearrangement
DNA
Eggs
Sequence Analysis
Chromosomes
Alleles
Enzymes

ASJC Scopus subject areas

  • Genetics

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The abnormal oocyte phenotype is correlated with the presence of blood transposon in Drosophila melanogaster. / Lavorgna, G.; Malva, C.; Manzi, A.; Gigliotti, S.; Graziani, F.

In: Genetics, Vol. 123, No. 3, 11.1989, p. 485-494.

Research output: Contribution to journalArticle

Lavorgna, G, Malva, C, Manzi, A, Gigliotti, S & Graziani, F 1989, 'The abnormal oocyte phenotype is correlated with the presence of blood transposon in Drosophila melanogaster.', Genetics, vol. 123, no. 3, pp. 485-494.
Lavorgna, G. ; Malva, C. ; Manzi, A. ; Gigliotti, S. ; Graziani, F. / The abnormal oocyte phenotype is correlated with the presence of blood transposon in Drosophila melanogaster. In: Genetics. 1989 ; Vol. 123, No. 3. pp. 485-494.
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