The activation of PPARγ by 2,4,6-Octatrienoic acid protects human keratinocytes from UVR-induced damages

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Abstract

Increasing attention is addressed to identify products able to enhance skin photoprotection and to prevent skin carcinogenesis. Several studies have demonstrated that the α-melanocyte stimulating hormone (αMSH), acting on a functional MC1R, provides a photoprotective effect by inducing pigmentation, antioxidants and DNA repair. We discovered a link between αMSH and the nuclear receptor Peroxisome Proliferator-Activated Receptor-γ (PPARγ), suggesting that some of the αMSH protective effects may be dependent on PPARγ transcriptional activity. Moreover, we demonstrated that the activation of PPARγ by the parrodiene 2,4,6-octatrienoic acid (Octa) induces melanogenesis and antioxidant defence in human melanocytes and counteracts senescence-like phenotype in human fibroblasts. In this study, we demonstrate that the activation of PPARγ by Octa exerts a protective effect against UVA- and UVB-induced damage on normal human keratinocytes (NHKs), the major target cells of UV radiation. Octa promotes the antioxidant defence, augments DNA repair and reduces the induction of proteins involved in UV-induced DNA damage response. Our results contribute to deepen the analysis of the αMSH/PPARγ connection and suggest perspectives for the development of new molecules and formulations able to prevent cutaneous UV damage by acting on the different skin cell populations through PPARγ activation.

Original languageEnglish
Article number9241
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - Aug 23 2017

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Peroxisome Proliferator-Activated Receptors
Keratinocytes
Melanocyte-Stimulating Hormones
Skin
Antioxidants
DNA Repair
Acids
Melanocytes
Pigmentation
Cytoplasmic and Nuclear Receptors
DNA Damage
octa-2,4,6-trienoic acid
Carcinogenesis
Fibroblasts
Radiation
Phenotype
Population
Proteins

ASJC Scopus subject areas

  • General

Cite this

@article{a73d371e09d84aca9f68dd13031915e1,
title = "The activation of PPARγ by 2,4,6-Octatrienoic acid protects human keratinocytes from UVR-induced damages",
abstract = "Increasing attention is addressed to identify products able to enhance skin photoprotection and to prevent skin carcinogenesis. Several studies have demonstrated that the α-melanocyte stimulating hormone (αMSH), acting on a functional MC1R, provides a photoprotective effect by inducing pigmentation, antioxidants and DNA repair. We discovered a link between αMSH and the nuclear receptor Peroxisome Proliferator-Activated Receptor-γ (PPARγ), suggesting that some of the αMSH protective effects may be dependent on PPARγ transcriptional activity. Moreover, we demonstrated that the activation of PPARγ by the parrodiene 2,4,6-octatrienoic acid (Octa) induces melanogenesis and antioxidant defence in human melanocytes and counteracts senescence-like phenotype in human fibroblasts. In this study, we demonstrate that the activation of PPARγ by Octa exerts a protective effect against UVA- and UVB-induced damage on normal human keratinocytes (NHKs), the major target cells of UV radiation. Octa promotes the antioxidant defence, augments DNA repair and reduces the induction of proteins involved in UV-induced DNA damage response. Our results contribute to deepen the analysis of the αMSH/PPARγ connection and suggest perspectives for the development of new molecules and formulations able to prevent cutaneous UV damage by acting on the different skin cell populations through PPARγ activation.",
author = "Enrica Flori and Arianna Mastrofrancesco and Daniela Kovacs and Barbara Bellei and Stefania Briganti and Vittoria Maresca and Giorgia Cardinali and Mauro Picardo",
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T1 - The activation of PPARγ by 2,4,6-Octatrienoic acid protects human keratinocytes from UVR-induced damages

AU - Flori, Enrica

AU - Mastrofrancesco, Arianna

AU - Kovacs, Daniela

AU - Bellei, Barbara

AU - Briganti, Stefania

AU - Maresca, Vittoria

AU - Cardinali, Giorgia

AU - Picardo, Mauro

PY - 2017/8/23

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